AZITHROMYCIN ATTENUATION OF INFLAMMATION IN A NON-INFECTIOUS ASTHMA

阿奇霉素减轻非感染性哮喘的炎症

• 批准号: 8361426
• 负责人: MICHAEL J WALTER
• 金额: $ 1.08万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361426
• 关键词: Anti-Inflammatory Agents; Anti-inflammatory; Antibiotic Therapy; Asthma; Azithromycin; BALB/cJ Mouse; Bronchoalveolar Lavage; Cells; Extrinsic asthma; Funding; Grant; Inflammation; Inflammation Mediators; Leukocytes; Macrolide Antibiotics; Mass Spectrum Analysis; Metaplasia; Mucous body substance; Mus; National Center for Research Resources; Ovalbumin; Principal Investigator; Property; Research; Research Infrastructure; Resources; Source; Testing; Time; United States National Institutes of Health; airway inflammation; allergic airway inflammation; antimicrobial; attenuation; biomedical resource; cost; intraperitoneal; mouse model; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Definitive conclusions regarding the anti-inflammatory effects of macrolide antibiotics for treatment of asthma are difficult to formulate since their beneficial effects may be related to their antimicrobial action. We hypothesized that azithromycin possesses distinct anti-inflammatory properties and tested this assumption in a non-infectious mouse model of allergic asthma. To induce allergic airway inflammation seven-week-old BALB/cJ mice underwent intraperitoneal ovalbumin sensitization on days 0 and 7 followed by intranasal challenge on day 14. Mice were treated with PBS or azithromycin on days 13 through 16 and on day 17 airway inflammation was assessed by quantifying leukocytes in the airway, expression of multiple inflammatory mediators in the bronchoalveolar lavage (BAL), and mucous cell metaplasia. In a separate set of experiments, azithromycin or PBS treatment were initiated after the ovalbumin challenge. Each experiment was repeated 3 times (a total of 9-11 mice in each group).

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 关于大花环抗生素对哮喘治疗的抗炎作用的明确结论很难制定，因为它们的有益作用可能与它们的抗菌作用有关。 我们假设阿奇霉素具有不同的抗炎特性，并在非感染的小鼠过敏性哮喘模型中测试了这种假设。 To induce allergic airway inflammation seven-week-old BALB/cJ mice underwent intraperitoneal ovalbumin sensitization on days 0 and 7 followed by intranasal challenge on day 14. Mice were treated with PBS or azithromycin on days 13 through 16 and on day 17 airway inflammation was assessed by quantifying leukocytes in the airway, expression of multiple inflammatory mediators in the支气管肺泡灌洗（BAL）和粘液细胞化生。在另一组实验中，卵霉素挑战后开始了阿奇霉素或PBS治疗。每个实验重复3次（每组总共有9-11只小鼠）。