PROTEIN QUANTIFICATION FOR MOUSE MODEL OF BREAST CANCER

乳腺癌小鼠模型的蛋白质定量

• 批准号: 8361420
• 负责人: RON BOSE
• 金额: $ 1.42万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361420
• 关键词: Data Analyses; Experimental Designs; Funding; Grant; Label; Mass Spectrum Analysis; Modeling; National Center for Research Resources; Phase; Principal Investigator; Proteins; Proteomics; Research; Research Infrastructure; Resources; Source; Transgenic Mice; United States National Institutes of Health; biomedical resource; cost; malignant breast neoplasm; mouse model; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This is a two phase project with the first being the optimization of label-free protein quantification for detecting differentially expressed proteins in a transgenic mouse model of breast cancer. The second phase is to use the quantification model to discover novel breast cancer effected proteins. Both phases will use the standard LC-MS/MS platform in use with the proteomics core, but will use statistical experimental design and a novel application of linear mixed models for data analysis.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 这是一个两相项目，首先是优化无标签蛋白 定量检测转基因小鼠中差异表达的蛋白 乳腺癌的模型。第二阶段是使用量化模型发现 新型乳腺癌影响蛋白质。这两个阶段将使用标准LC-MS/MS 与蛋白质组学核心一起使用的平台，但将使用统计实验设计和 线性混合模型的新颖应用用于数据分析。