IDENTIFYING THE PATH OF THE RNA PRIMER AROUND A SIMPLE RNA POLYMERASE

识别简单 RNA 聚合酶周围 RNA 引物的路径

• 批准号: 8362189
• 负责人: CHANGGONG LI
• 金额: $ 0.03万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362189
• 关键词: Binding Sites; Complex; DNA-Directed RNA Polymerase; Ensure; Funding; Grant; Hour; Individual; National Center for Research Resources; Polymerase; Polynucleotide Adenylyltransferase; Principal Investigator; Proteins; RNA; RNA Polymerase I; RNA primers; Radiation; Research; Research Infrastructure; Resources; Source; Structure; Surface; Tail; Time; United States National Institutes of Health; Vaccinia virus; Work; cost; flexibility; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Vaccinia virus poly(A) polymerase (VP55) is the only RNA polymerase known to translocate with respect to its RNA primer, raising a paradox: Which is more flexible: The polymerase or the primer? The protein has two known modes of action, namely the processive synthesis of 30 nt tails and, as a heterodimer with its processivity factor (VP39), the semi-processive synthesis of 200 ? 300 nt tails. Past, collaborative studies, have yielded crystal structures for VP39, and for the VP55-VP39 heterodimer. The most important, yet absent information is a structural understanding of the heterodimer-primer complex. By using the beam time of a colleague, a full-time crystallographer in the Gershon lab has progressed markedly in finding binding sites for short RNA segments on the polymerase surface. Although this is ground-breaking, original work for a poly(A) polymerase, 3 hours of beam time every 2 months provides inadequate progress in this NIH-funded work to ensure grant renewal. Beam time is the major bottleneck, hence the current request for individual, regular beam time for this crystallographic work.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 Vacinia病毒聚（A）聚合酶（VP55）是唯一已知相对于其RNA底漆的RNA聚合酶，提高了悖论：哪个更灵活：聚合酶或底漆？该蛋白质具有两种已知的作用模式，即30 nt尾巴的过程合成，并且作为具有其加工性因子的异二聚体（VP39），是200的半配学合成？ 300 nt尾巴。过去的协作研究已经为VP39和VP55-VP39异二聚体产生了晶体结构。最重要但缺乏信息是对异二聚体络合物复合物的结构理解。通过使用同事的光束时间，Gershon Lab中的一名全职晶体学家在发现聚合酶表面上短RNA片段的结合位点方面取得了显着发展。尽管这是开创性的，是聚（A）聚合酶的原始作品，但每2个月的3个小时的光束时间在这项NIH资助的工作中提供了不足的进度，以确保授予续签。光束时间是主要的瓶颈，因此当前对此晶体学工作的单个常规光束时间请求。