RETROVIRUS RNA

逆转录病毒RNA

• 批准号: 8361085
• 负责人: MICHAEL FINLEY SUMMERS
• 金额: $ 6.56万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361085
• 关键词: Attention; Crying; Dimerization; Electron Microscopy; Funding; Genome; Grant; Human; Longevity; Modeling; Moloney Leukemia Virus; Murine leukemia virus; National Center for Research Resources; Nucleotides; Principal Investigator; RNA; Research; Research Infrastructure; Resources; Retroviridae; Signal Transduction; Source; Structure; United States National Institutes of Health; Viral; Virus Assembly; cost; dimer; gene therapy; macromolecule; stem; vector

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Moloney Murine Leukemia Virus (MLV) is a simple retrovirus that has received considerable attention as a model for virus assembly, and is currently the most widely used vector in human gene therapy trials. Like most retroviruses, MLV packages two copies of its genome, proper packaging of which is critical for viral lifespan and infectivity. A region of about 75 nucleotides, which is believed to act as a 'core encapsidation signal,' has been identified within the MLV genome. This region forms a double hairpin motif, where two sets of two stem loop structures are capable of forming a 46kDa dimer via 'kissing interactions' at the tips of the loops. We propose to use cry-EM to determine the structure of ?core encapsidation signal? which will provide the precise mechanisms of genome dimerization and packaging.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 莫洛尼鼠白血病病毒 (MLV) 是一种简单的逆转录病毒，已被 作为病毒组装的模型受到相当大的关注，并且是目前使用最广泛的 人类基因治疗试验中的载体。与大多数逆转录病毒一样，MLV 包装了其两个副本 基因组，其正确包装对于病毒的寿命和传染性至关重要。一个地区 大约 75 个核苷酸，被认为充当“核心衣壳化信号”，已被 在 MLV 基因组中鉴定。该区域形成双发夹图案，其中两组 两个茎环结构能够通过“接吻相互作用”形成 46kDa 二聚体 循环的尖端。 我们建议使用cry-EM来确定?核心的结构 衣壳化信号？这将提供基因组二聚化的精确机制 包装。