KV CHANNEL IN LIPID MEMBRANES (2D CRYSTALS OF KVAP)

脂质膜中的 KV 通道（KVAP 的二维晶体）

• 批准号: 8361108
• 负责人: Qiu-Xing Jiang
• 金额: $ 1.23万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361108
• 关键词: Electron Microscopy; Funding; Goals; Grant; Lipids; Membrane Lipids; Molecular Conformation; National Center for Research Resources; Potassium; Principal Investigator; Proteins; Research; Research Infrastructure; Resolution; Resources; Source; Structure; United States National Institutes of Health; cost; macromolecule; three dimensional structure; voltage

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This project will be aimed at obtaining structures of a voltage-gated potassium (Kv) channel in lipid membranes. There are at least two distinct conformations for a Kv channel and we want to obtain both structures at 5-9 Angstrom resolution so that we can deduce the secondary structures. If conditions permit, we will pursue structures at higher resolutions that will reveal the structural details for the strong lipid-protein interaction that has been demonstrated to be critical for voltage-gating. The immediate goal is to obtain the 3D structure of a 2D crystal form that is currently available.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 该项目将旨在获取脂质膜中电压门控钾（KV）通道的结构。 KV通道至少有两个不同的构象，我们希望以5-9的埃斯特罗姆分辨率获得这两个结构，以便我们可以推断出二级结构。如果条件允许，我们将在更高的分辨率下追求结构，这些结构将揭示强烈的脂质蛋白相互作用的结构细节，这已被证明对电压门控至关重要。直接目标是获得当前可用的2D晶体形式的3D结构。