TETRALOOP RECEPTOR RNA STRUCTURAL INVESTIGATION

四环受体 RNA 结构研究

• 批准号: 8361194
• 负责人: Samuel E Butcher
• 金额: $ 1.36万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361194
• 关键词: Bacteriophage Pf1; Binding; COSY; Functional RNA; Funding; Grant; Investigation; Kinetics; Measures; Mediating; NOESY; National Center for Research Resources; Principal Investigator; RNA; Research; Research Infrastructure; Residual state; Resources; Sampling; Source; Structure; TOCSY; Temperature; United States National Institutes of Health; Work; cost; receptor; research study; Bacteriophage Pf1; Binding; COSY; Functional RNA; Funding; Grant; Investigation; Kinetics; Measures; Mediating; NOESY; National Center for Research Resources; Principal Investigator; RNA; Research; Research Infrastructure; Residual state; Resources; Sampling; Source; Structure; TOCSY; Temperature; United States National Institutes of Health; Work; cost; receptor; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We are investigating the structure of the free tetraloop receptor. The tetraloop receptor is a commonly occurring motif that mediates tertiary folding of large, non-coding RNAs. We are investigating the tetraloop receptor structure as a function of temperature and ionic conditions. Previous work shows that the tetraloop receptor undergoes a conformational change upon tetraloop binding. We are also investigating the kinetics of this conformational change by NMR. NMR experiments that we will need to collect include: 1D (1H), 2D NOESY, 2D 1H-15N HSQC (normal and TROSY enhanced), 2D 1H-15N HNN COSY, 2D 1H-13C HSQC (normal and TROSY enhanced), 2D NOESY, 2D 1H,13C filter/edit NOESY, 3D 1H(13C)1H NOESY-HSQC, 3D 1H(13C)1H HCCH TOCSY and 3D 1H(13C)1H HCCH COSY. Residual dipolar couplings will be measured for samples in both isotropic and partially aligned states (via addition of Pf1 phage).

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 我们正在研究游离四边形受体的结构。 四边形受体是通常发生的基序，可介导大型非编码RNA的三级折叠。 我们正在研究四边形受体结构，这是温度和离子条件的函数。 先前的工作表明，四边形受体在四边形结合后发生构象变化。 我们还正在研究NMR的这种构象变化的动力学。 我们需要收集的NMR实验包括：1d（1h），2d Noesy，2d 1H-15N HSQC（正常和果岭增强），2d 1h-15n Hnn Cozy，2d 1H-13C HSQC（正常和trosy and anc）（正常和TROSY增强），2d Noesy，2d noes，2d 1H，1H，13c pilter/noes noes noes noesy（13c）1（13c）1（13c），3d 1（3d c. 1 y. 3 c），3d。 NOESY-HSQC，3d 1H（13C）1H HCCH TOCSY和3D 1H（13C）1H HCCH COZY。将测量各向同性和部分比对态中的样品（通过添加PF1噬菌体）测量残留的偶极耦合。