COBRE: LSU: LIPIDOMIC CORE RESOURCE MODULE

COBRE：LSU：脂质核心资源模块

• 批准号: 8359599
• 负责人: Song Hong
• 金额: $ 17.02万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-02-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8359599
• 关键词: Atmospheric Pressure; Cells; Centers of Research Excellence; Chemicals; Derivation procedure; Electrospray Ionization; Experimental Designs; Funding; Grant; High Pressure Liquid Chromatography; Immunoassay; Ions; Lipids; Liquid Chromatography; Louisiana; Mentors; National Center for Research Resources; Neurosciences; Participant; Phase; Principal Investigator; Process; Radio; Research; Research Infrastructure; Research Personnel; Resources; Sampling; Signal Transduction; Solid; Source; Techniques; Tissues; Training; United States National Institutes of Health; base; computerized data processing; cost; flexibility; ionization; lipid mediator; programs; tandem mass spectrometry

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This core module provides guidance and advice for experimental design to study lipid signaling. This core also assists and trains COBRE investigators on tissue cell sampling, processing, and extraction, followed by lipid purification and/or derivation for structural characterization of putattive lipid messengers using tandem liquid chromatography-photodiode array-electrospray ionization-tandem MS/MS-based lipidmoic analysis. Liquid chromatography-photodiode array-electrospray ionization-atmospheric pressure chemical ionization-tandem mass spectrometry is performed on state-of-the-art triple quadrupole LC-MS, or an ion trap, thus providing powerful and flexible quantitative analysis and structural characterization of lipid mediators. other separation techniques are also available, such as HPLC, GLC, TLC, and solid-phase extrction, as well as analysis by radiometric, radio-immunoassay, or fluorometric applications. COBRE participants receive technical support and assistance on data processing and interpretation.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 该核心模块为研究脂质信号传导提供了指导和建议。该核心还可以通过使用TANDEM液体色谱 - 光谱阵列阵列 - 基于iOctRayization-TandAndem-Tandandem MSS/MS基于MS/MS基于MS/MS基于LipidMoic分析的分析来帮助和培训COBRE研究人员就组织细胞采样，加工和提取的组织采样，加工和提取。液相色谱 - 光谱阵列阵列 - 电喷雾 - 大气压化学电离 - 化学电离串联质谱法是在最先进的三倍四极杆LC-MS上或离子陷阱上进行的，因此提供了强大而柔性的定量分析和柔性的定量分析和脂质媒介物的结构表征。还提供了其他分离技术，例如HPLC，GLC，TLC和固相习惯，以及通过辐射，放射性，免疫测定或荧光测定应用分析。 COBRE参与者在数据处理和解释方面获得了技术支持和帮助。