INBRE: UOFL: GENOMICS CORE

INBRE：UOFL：基因组学核心

• 批准号: 8360098
• 负责人: NIGEL G.F. COOPER
• 金额: $ 30.08万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360098
• 关键词: Area; Biomedical Research; Certification; Client; Clinical; Core Facility; Data Analyses; Funding; Genes; Genetic screening method; Grant; Health Sciences; Housing; Human; Human Resources; Institution; Location; MicroRNAs; Molecular Profiling; National Center for Research Resources; Ozone; Patients; Principal Investigator; Process; Research; Research Infrastructure; Resources; Rodent; Sampling; Services; Source; System; Training; Translational Research; United States National Institutes of Health; Universities; Work; base; cost; design; instrumentation; square foot

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The shared, centralized research core facility is designed to provide sequencing and gene-micro-array services to the University of Louisville and its KBRIN/INBRE affiliated institutions. In September 2009, the UofL Microarray Facility moved to the new Clinical Translational Research Building (CTRB), opened in August 2009 on the same Health Science Center Campus. This building provides state-of-the-art new and expanded lab space for the Facility. The new facility is approx. 1500 square feet, which includes four large lab areas with benches, a separate room for processing ozone sensitive Agilent arrays and a pre-PCR room. The facility houses an Agilent Microarray Scanner G2505B, Workstation, and Hybridization Oven G2545A, an Affymetrix Microarray System, including an Affymetrix GCS 3000 Scanner 7G, two Fluidics Stations 450 and a Hybridization Oven 640, a Beckman CEQ 8000 Sequencer, an Agilent Bioanalyzer 2000, a Nanodrop ND-1000 and three Applied Biosystems PCR Thermocyclers 9700. This core hosts specialized training on an on going basis. Most importantly, the move allowed to physically merge the Affymetrix and Agilent components of the Facility and their respective personnel into one centralized Facility location, with ample bench space for all hardware and instrumentation, work and storage space, and desk areas for all personnel. The Facility is in the process of obtaining CLIA certification for the processing of standardized CGH genetic tests on clinical patient samples using the Agilent platform. As expansion of the services offered, microRNA expression profiling on human and rodent samples using either the Agilent or Affymetrix array platforms was introduced and is now routinely performed. Also, Yinlu Chen, M.S., Sr. Research Analyst, was hired in Dec 2009 as addition to the Facility personnel to support client data analyses onsite.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 共享的集中研究核心设施旨在为路易斯维尔大学及其KBRIN/INBRE附属机构提供测序和基因微阵列服务。 2009年9月，UOFL微阵列设施搬到了2009年8月在同一个健康科学中心校园开业的新临床转化研究大楼（CTRB）。该建筑为该设施提供了最新的新实验室空间。新设施约为1500平方英尺，其中包括四个带长凳的大型实验室区域，一个单独的处理臭氧敏感敏捷阵列的房间和一个预PCR室。 The facility houses an Agilent Microarray Scanner G2505B, Workstation, and Hybridization Oven G2545A, an Affymetrix Microarray System, including an Affymetrix GCS 3000 Scanner 7G, two Fluidics Stations 450 and a Hybridization Oven 640, a Beckman CEQ 8000 Sequencer, an Agilent Bioanalyzer 2000, a Nanodrop ND-1000和三个Applied Biosystems PCR热循环器9700。此核心托管专门的培训。 最重要的是，此举使该设施的Affymetrix和Agilent组件及其各自的人员可以将其物理合并为一个集中式设施位置，并为所有硬件和仪器，工作和存储空间以及所有人员提供了足够的长凳空间。 该设施正在获得CLIA认证，用于使用Agilent平台在临床患者样品上处理标准化的CGH基因检测。 随着提供的服务的扩展，引入了使用Agilent或Affymetrix阵列平台对人和啮齿动物样品上的microRNA表达谱分析，现在常规执行。 此外，Yinlu Chen，M.S。，Sr。研究分析师于2009年12月被聘用，作为设施人员的补充，以支持现场客户数据分析。