AN AFRICAN GREEN MONKEY MODEL OF DENGUE HEMORRHAGIC FEVER

登革出血热非洲绿猴模型

• 批准号: 8358010
• 负责人: Katharine Nina Bossart
• 金额: $ 5.4万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8358010
• 关键词: Animal Model; Animals; Biological; Biological Assay; Cercopithecus aethiops; Cercopithecus pygerythrus; Cessation of life; Clinical; Coagulation Process; Data; Dengue; Dengue Hemorrhagic Fever; Dengue Virus; Development; Disease; Exanthema; Fever; Funding; Goals; Grant; Human; Immune; Individual; Infection; Mediating; Modeling; Monitor; National Center for Research Resources; New England; Pathway interactions; Pilot Projects; Predisposition; Primate Diseases; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Sampling; Source; Symptoms; Time; United States National Institutes of Health; Viral Load result; base; cost; drug development; nonhuman primate; therapeutic vaccine

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Dengue virus infects over 50 million individuals each year causing significant disease and approximately 50,000 deaths. Currently, there are no animal models for Dengue fever and Dengue hemorrhagic fever which mimic the disease seen in humans and consequently, vaccine and therapeutic drug development have progressed slowly. In the current project we will examine the susceptibility of African green monkeys (Cercopithecus aethiops) to Dengue virus. The goal of this project is to develop a new animal model of Dengue virus that closely parallels the disease seen in humans, including the development of Dengue hemorrhagic fever. The current study is a collaborative pilot study that uses only a small number of animals. For these studies we will use a new isolate of dengue virus that originated in nonhuman primates and caused dengue hemorrhagic fever in a human. This new dengue isolate will be used to infect nonhuman primates and disease will be monitored for 21 days following infection, including outward clinical symptoms, skin rashes or irregularities and fever. Biological samples will be collected at various time points and viral loads, immune parameters and coagulation pathways will be evaluated using standard microbiological and clinical assays. By analyzing all of the disease symptoms and clinical/microbiological data, we will be able to determine if Cercopithecus aethiops are a viable host to develop a new model of severe dengue-mediated disease that mimics the disease seen in humans.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 登革热病毒每年感染超过5000万人，导致重大疾病和约50,000人死亡。目前，没有用于登革热和登革热大量发烧的动物模型，它们模仿了人类中的疾病，因此，疫苗和治疗药物发育进展缓慢。在当前的项目中，我们将研究非洲绿猴（Cercopithecus aethiops）对登革热病毒的敏感性。该项目的目的是开发一种新的登革热动物模型，该模型与人类中所见的疾病相似，包括发展登革热大量发烧。当前的研究是一项合作试验研究，仅使用少量动物。 在这些研究中，我们将使用起源于非人类灵长类动物的登革热病毒的新分离物，并引起人类登革热的出血热。 这种新的登革热分离株将用于感染非人类灵长类动物，并在感染后21天监测疾病，包括外向临床症状，皮疹或不规则性和发烧。 将在各个时间点和病毒载荷，免疫参数和凝结途径中收集生物样品，并使用标准的微生物学和临床测定法进行评估。 通过分析所有疾病症状和临床/微生物学数据，我们将能够确定Cercopithecus aethiops是否是可行的宿主，可以开发出一种新的严重登革热介导的疾病模型，以模仿人类在人类中看到的疾病。