Discovering Genome Evolution in a Model System for Plant Ecological Genomics

在植物生态基因组学模型系统中发现基因组进化

• 批准号: 8261995
• 负责人: Lex Evan Flagel
• 金额: $ 0.95万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-15 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8261995
• 关键词: Affect; Animal Model; Arabidopsis; Biological Models; Biology; Chromosomal Duplication; Complex; Doctor of Philosophy; Drosophila genus; Ecology; Evolution; Frequencies; Future; Gene Expression; Gene Mutation; Genetic; Genome; Genomics; Genotype; Goals; Habitats; Human; Inbreeding; Individual; Learning; Link; Maize; Maps; Mimulus; Molecular Evolution; Mutation; Nucleotides; Phenotype; Plants; Play; Population; Population Genetics; Relative (related person); Research; Resolution; Rewards; Role; Shapes; Source; Structure; Study models; Surveys; System; Technology; Time; Variant; Work; base; career; experience; genetic evolution; human disease; insertion/deletion mutation; novel; skills; structural genomics; theories; trait

DESCRIPTION (provided by applicant): We propose to study chromosomal structural variation in the Mimulus genome. This work will focus on the Mimulus guttatus species complex, a diverse group of plants with a broad range of phenotypic diversity and habitat specialization. The primary goal of this study will be to make a first attempt at estimating the extent of structural variation within the Mimulus guttatus species complex. The long-term goal of the proposed project is serve as a starting point to determine what role, if any, structural variation has played in the evolution of ecological traits with this complex. Because of the significant natural variation found within these species and their utility and wide-spread use in evolutionary and ecological studies, this project will open up exciting and novel research directions. Studies in humans have shown that there are considerable levels of genomic structural variation within individuals. A small number of these structural variants have been connected to human disease, however, on the whole we have a very limited understanding of the impact genomic structural variation has had on organismal phenotype. The long-term goal of our study is to tackle this problem by connecting these genomic changes to phenotypic evolution. I am very excited about the possibilities the future holds for understanding the genetic basis of ecologically important traits in natural systems. My long-term career goal is to integrate the fast moving field of genomics with evolutionary biology, ecology, and genetics to study evolution and adaptation in natural plant systems. These problems are complex, and they require significant background in each of the fields mentioned above. The proposed project will give me the opportunity to develop my skills in each of these fronts. Specifically, the project will involve the integration of cutting edge genomic technology with evolutionary analyses in Mimulus a model system for evolutionary and ecological studies. The single major skill I hope to develop through this proposal is the ability to study evolution and genetics on multiple levels, from the genome of an individual, to a population, and beyond to a diversification of species. During my PhD research I have primarily focused on genomics, molecular evolution, and population genetics. These form a core set of skill from which I can build. Portions of the proposed project I will enhance these core skills, for example the fine-scale analysis of multiple Mimulus genomes. Other portions of the project will develop totally new skills, for example linking genomic structural variation to the evolution of complex traits in natural populations. I believe that learning new theories and skills, while at the same time building on my existing strengths is the ideal path toward a productive and rewarding postdoctoral research experience.

描述（由申请人提供）：我们建议研究模拟物基因组中的染色体结构变异。这项工作将集中于Mimulus guttatus物种复合物，这是一组具有广泛表型多样性和栖息地专业化的植物。这项研究的主要目的是首次尝试估计Mimulus Guttatus物种复合物内结构变化的程度。提出的项目的长期目标是确定与这种复合物在生态特征的演变中起着什么作用（如果有的话）的起点。由于这些物种中发现的自然变化及其效用以及在进化和生态学研究中广泛使用，因此该项目将打开令人兴奋和新颖的研究方向。在人类中的研究表明，个体内部基因组结构变化水平相当大。这些结构变体中有少数与人类疾病有关，但是，总的来说，我们对基因组结构变异对有机体表型的影响的理解非常有限。我们研究的长期目标是通过将这些基因组变化与表型进化联系起来来解决这个问题。我对未来了解自然系统中生态重要特征的遗传基础的可能性感到非常兴奋。我的长期职业目标是将基因组学的快速运动领域与进化生物学，生态学和遗传学相结合，以研究天然植物系统中的进化和适应。这些问题很复杂，它们需要上述每个字段中的重要背景。拟议的项目将使我有机会在这些方面发展自己的技能。具体而言，该项目将涉及尖端基因组技术与Mimulus中的进化分析的整合，用于进化和生态研究的模型系统。我希望通过该提案发展的一项主要技能是能够在多个层面上研究进化和遗传学的能力，从个体的基因组到人群，再到物种多样化的能力。在我的博士研究期间，我主要集中于基因组学，分子进化和人群遗传学。这些构成了我可以从中构建的一组核心技能。拟议项目的一部分我将增强这些核心技能，例如对多个Mimulus基因组的精细分析。该项目的其他部分将发展全新的技能，例如将基因组结构变化与自然种群中复杂性状的演变联系起来。我相信学习新的理论和技能，同时建立在我现有的优势上是迈向富有成效和有意义的博士后研究经验的理想途径。