SURFACE ENHANCED FLUORESCENCE NANOTAGS FOR ULTRASENSITIVE MULTIPLEXED ASSAY SYST

用于超灵敏多重检测系统的表面增强荧光纳米标记

• 批准号: 8341466
• 负责人: DAVID SEBBA
• 金额: $ 20万
• 依托单位: NANOCOMPOSIX, INC.
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-15 至 2012-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8341466
• 关键词: Antibodies; Antigens; Binding; Biological Assay; Blinking; Cancer Detection; Cancer Diagnostics; Chemistry; Detection; Dot Immunoblotting; ERBB2 gene; Engineering; Enzyme-Linked Immunosorbent Assay; Excision; Exhibits; Fluorescence; Fluorescence Spectroscopy; Fluorescent Antibody Technique; Fluorescent Probes; Glutamate Carboxypeptidase II; Label; Link; Location; Malignant Neoplasms; Metals; Methods; Nanosphere; Nanotechnology; Optics; Performance; Quantum Dots; Sensitivity and Specificity; Signal Transduction; Silicon Dioxide; Silver; Solutions; Specificity; Surface; Survival Rate; TP53 gene; Testing; Thick; Time; Toxic effect; antibody engineering; base; fluorophore; improved; light emission; nanoengineering; nanoparticle; particle; plasmonics

Late detection is the primary cause of poor survival rates from cancer. Nanotechnology-enabled assays can detect early markers of cancer with much higher sensitivity than current detection methods. Existing assays use fluorescent probes with organic fluorophores or quantum dots, both of which have limitations. Organic fluorophores have limited brightness and photostability, reducing sensitivity and effective signal quantitation. Quantum dots exhibit toxicity and irregular blinking as well as broad emission spectra which reduces the level of multiplexing possible. We will develop a new class of ultrabright and ultrastable fluorescence probes, Surface Enhanced Fluorescence (SEF) Nanotags, to improve the sensitivity of fluorescence-based cancer detection. Nanoengineering of the SEF-nanotags to place fluorophores near the surface of plasmonic nanoparticles increases the light emission by up to a factor of 100X and significantly increases probe photostability. SEF probes will be functionalized with antibodies engineered to target cancer markers. The SEF nanotags will interrogate an analyte-containing solution and be detected on a microarray of complementary chemotropes. The brightness, photostability, and detection sensitivity of the SEF-nanotags will be compared to existing probes. These ultrabright and ultrastable SEF nanotags are easily adapted to existing fluorescence-based cancer diagnostics with improved sensitivity.

晚期发现是癌症存活率低的主要原因。纳米技术支持的检测可以检测癌症的早期标志物，其灵敏度比当前的检测方法高得多。现有的检测使用带有有机荧光团或量子点的荧光探针，这两种方法都有局限性。有机荧光团的亮度和光稳定性有限，降低了灵敏度和有效信号定量。量子点表现出毒性和不规则闪烁以及宽发射光谱，这降低了可能的复用水平。我们将开发一类新型超亮和超稳定荧光探针，即表面增强荧光（SEF）纳米标签，以提高基于荧光的癌症检测的灵敏度。 SEF 纳米标签的纳米工程将荧光团放置在等离激元纳米颗粒表面附近，可将光发射增加高达 100 倍，并显着提高探针的光稳定性。 SEF 探针将通过针对癌症标记物而设计的抗体进行功能化。 SEF 纳米标签将询问含有分析物的溶液，并在互补趋化剂微阵列上进行检测。 SEF 纳米标签的亮度、光稳定性和检测灵敏度将与现有探针进行比较。这些超亮和超稳定的 SEF 纳米标签可以轻松适应现有的基于荧光的癌症诊断，并提高灵敏度。