Role of Gq/11-alpha in metabolic regulation

Gq/11-alpha 在代谢调节中的作用

• 批准号: 8349964
• 负责人: Lee Weinstein
• 金额: $ 19.89万
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8349964
• 关键词: Alleles; Eating; Energy Metabolism; Environment; Enzymes; GTP-Binding Proteins; Genes; Goals; Hormones; House mice; Hypothalamic structure; Life; Metabolic; Morbid Obesity; Mus; Neuraxis; Neurotransmitters; Peripheral; Phenotype; Phospholipase C; Play; Regulation; Role; Signal Transduction; Tissues; Transgenes; Transgenic Mice; Underweight; Weight Gain; extracellular; glucose metabolism; paraventricular nucleus; promoter; receptor coupling; recombinase

To date we have generated mice with Gq/11-alpha deficiency in the paraventricular nucleus of the hypothalamus (PVN) using Sim1-cre transgenic mice. Preliminary findings show that these mice are underweight in the first month of life but then do develop severe obesity. However when the mice are housed individually they do not gain weight, suggesting that environment may imppact on the phenotype. Studies are underway to determine to what extent this is due to excess food intake vs. reduced energy expenditure.

迄今为止，我们使用SIM1-CRE转基因小鼠在下丘脑（PVN）的室室核中产生了具有GQ/11-Alpha缺乏症的小鼠。初步发现表明，这些小鼠在生命的第一个月中体重不足，但随后会出现严重的肥胖症。但是，当小鼠分别饲养时，它们不会增加体重，这表明环境可能会在表型上添加。正在进行研究以确定这是由于食物摄入过量与减少能量消耗所致。