NEIBank: Est Analysis And Bioinformatics For Ocular Genomics

NEIBank：眼部基因组学的 Est 分析和生物信息学

• 批准号: 8339760
• 负责人: graeme j wistow
• 金额: $ 27.21万
• 依托单位: NATIONAL EYE INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8339760
• 关键词: Animal Model; Bioinformatics; Collaborations; Complementary DNA; Complex; Conjunctival Pterygium; Custom; DNA; DNA Microarray Chip; Data; Data Analyses; Databases; Development; Disease; Ear; Expressed Sequence Tags; Eye; Eye diseases; Future; Gene Expression; Genes; Genetic; Genome; Genomics; Glaucoma; Human; Hybrids; Informatics; Libraries; Modeling; Molecular; Molecular Profiling; Mus; Optic Nerve; Oryctolagus cuniculus; Pattern; Protein Analysis; Research; Research Personnel; Resources; Retina; Retinal Cone; Sensory; Spermophilus; System; Taste Buds; Tissues; Trabecular meshwork structure; Vision; Yeasts; cDNA Arrays; design; human data; improved; insight; mouse model; research study; response; tool

Many human eye (and also ear) tissues and those of important animal models have now been analyzed. In addition NEIBank collaborations have added data on other sensory tissues, ear and taste bud. Recent eye additions include human optic nerve and human pterygium. This DNA resource has been used to create cDNA microarrays with 13,000 non-redundant human eye expressed sequences and 6500 sequences from mouse eye. These are being used in analysis of human cellular responses and genetically modified mouse models. A powerful addition to the informatics tools for eye research is EyeBrowse, a dedicated genome browser for eye-expressed genes, produced in collaboration with CIT. http://eyebrowse.cit.nih.gov/genome/. Major additions are a unique annotated database of human eye disease genes and databases of candidate eye disease regions of the genome. These are displayed in EyeBrowse along with all known human eye-derived cDNAs and SAGE data, indicating expression profiles specific to eye. A recent addition is a track for SAGE data for human Trabecular Meshwork with relevance to glaucoma. NEIBank analyses provide detailed insights into the expression of genes in eye tissues of several important animal models. Most recently this includes data on the ground squirrel retina, which is a model for a cone-related function and disease in human eye. Data from this analysis are being used to design a custom microarray for future experiments.

现在已经分析了许多人眼（以及耳朵）组织和重要动物模型的组织。此外，Neibank的合作增加了有关其他感觉组织，耳朵和味蕾的数据。最近的眼睛添加包括人视神经和人类翼状。该DNA资源已用于创建具有13,000个非冗余人眼表达序列和6500个序列的cDNA微阵列。这些用于分析人类细胞反应和基因修饰的小鼠模型。 与CIT合作生产的专用基因组浏览器Eykrowse是Eykrowse的强大补充。 http://eyebrowse.cit.nih.gov/genome/。 主要补充是人眼病基因的独特注释数据库和基因组候选眼病区域的数据库。这些显示在眉毛中，以及所有已知的人眼源性cDNA和鼠尾草数据，表明眼睛特有的表达曲线。最近的一个增加是人类小梁网的鼠尾草数据，与青光眼相关。 Neibank分析提供了一些关于几种重要动物模型眼组织中基因表达的详细见解。最近，这包括地下松鼠视网膜上的数据，这是人眼中与锥体相关功能和疾病的模型。该分析的数据用于设计自定义微阵列以供将来的实验。