Evaluation of athero-protective role of blue-green algae

蓝绿藻的动脉粥样硬化保护作用评价

基本信息

批准号：
7939944
负责人：
Ji-Young Lee
金额：
$ 17.89万
依托单位：
UNIVERSITY OF CONNECTICUT STORRS
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-30 至 2013-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7939944
关键词：
Address Alanine Anatoxins Animal Model Antiatherogenic Area Atherosclerosis Basic Science Binding Proteins Biological Factors Biological Process Cardiovascular Diseases Cause of Death Cholesterol Clinical Clinical Trials Communities Complementary and alternative medicine Consumption Controlled Environment Country Cyanobacterium Data Development Diet Dietary Supplementation Dyslipidemias Ensure Evaluation Food Foundations Gene Expression Genes Goals Harvest Healed Health Herbal Medicine Human In Vitro Indigenous Investigation Laboratories Lipids Marketing Mission Molecular Mus National Center for Complementary and Alternative Medicine Neurotoxins Nostoc Outcome Pathway interactions Plasma Prevention Preventive Production Property Public Health Recording of previous events Regulatory Element Research Research Personnel Risk Factors Role Safety Saxitoxin Science Source Spirulina preparation Sterols Supplementation Testing Therapeutic Toxic effect Toxin Training Triglycerides United States Work atherogenesis base cardiovascular disorder prevention cardiovascular disorder risk commune dietary supplements efficacy testing healing heart disease prevention hepatotoxin in vivo innovation insight interest meetings microcystin prevent response

项目摘要

DESCRIPTION (provided by applicant): There is a fundamental gap between the practical use of blue-green algae (BGA) as complementary and alternative medicine (CAM) substances and our understanding of their safety and biological functions. The long-term research goal is to identify and develop natural sources for CAM for promoting optimal health. The objectives of this project are to assess safety and athero-protective property of a newly cultivated BGA species Nostoc commune var. sphaeroides Kutzing (NO) and to elucidate the molecular mechanisms underlying the athero-protection. Spirulina plantensis (SP) will be used as a control BGA for comparison because it is the most common cultured BGA species that is known to be free of algal toxins. Our central hypothesis is that NO is a safe CAM substance that have hypocholesterolemic (cholesterol-lowering) and hypotriglyceridemic (triglyceride-lowering) properties, ultimately retarding the progress of atherosclerosis development. Guided by strong preliminary data, this hypothesis will be tested by pursuing three specific aims: 1) Evaluate safety of BGA in vivo and in vitro; 2) Assess lipid-lowering effects of BGA and identify molecular mechanisms; and 3) Determine anti-atherogenic roles of BGA in mice. The proposed work is innovative in that no study exists to assess safety of BGA supplementation in vivo and to mechanistically evaluate their efficacy in athero-protection using an animal model of atherosclerosis. Additionally, it is the first study that examines biological functions of newly cultivated NO in comparison with cultivated SP that is currently consumed as a dietary supplement. Cultivation of BGA can circumvent controversial health issues on BGA as quality and toxin contamination can be tightly controlled during production. In this regard, cultivated NO and SP present their worth for investigation on biological functions over naturally-harvested BGA species. With respect to expected outcomes, collective works proposed in specific aims 1-3 are expected to provide insights into BGA toxicity and athero-protective property with the elucidation of molecular mechanisms. Such results will have an important positive impact on public's health by providing scientific foundation for human clinical trials to test efficacy of BGA as effective and reliable CAM substances to reduce the risk of CVD. Therefore, the proposed research fits well to the mission of NCCAM "to explore complementary and alternative healing practices in the context of rigorous science, training CAM researchers, and disseminating authoritative information to the public and professional communities." PUBLIC HEALTH RELEVANCE: Blue-green algae products have been introduced as a dietary supplement to meet increasing public's interest in using natural products for preventive and therapeutic means for heart disease prevention. Safety and biological functions of various BGA species, however, are not properly studied. This project will assess safety and athero-protective property of newly cultivated blue-green alga Nostoc commune var. sphaeroides Kutzing together with Spirulina plantensis, which is the most common cultivated BGA in the current dietary supplement market. The project addresses an important yet under-investigated area of basic research on complementary and alternative medicine (CAM) substances that will have a positive impact on public's health by providing scientific foundation for human clinical trials to test efficacy of BGA as effective and reliable CAM substances to reduce the risk of cardiovascular disease.

描述（由申请人提供）：蓝绿藻（BGA）作为补充和替代医学（CAM）物质的实际使用与我们对其安全性和生物功能的理解之间存在根本差距。长期研究目标是识别和开发 CAM 天然来源，以促进最佳健康。该项目的目标是评估新培育的 BGA 物种发菜 (Nostoc commune var.) 的安全性和动脉粥样硬化保护特性。 sphaeroides Kutzing (NO) 并阐明动脉粥样硬化保护的分子机制。植物螺旋藻 (SP) 将用作对照 BGA 进行比较，因为它是最常见的培养 BGA 物种，且已知不含藻类毒素。我们的中心假设是，NO 是一种安全的 CAM 物质，具有降低胆固醇（降低胆固醇）和降低甘油三酯（降低甘油三酯）的特性，最终延缓动脉粥样硬化的进展。在强有力的初步数据的指导下，这一假设将通过追求三个具体目标来检验：1）评估BGA的体内和体外安全性； 2）评估BGA的降脂作用并确定分子机制； 3) 确定 BGA 在小鼠体内的抗动脉粥样硬化作用。这项工作的创新之处在于，目前尚无研究评估 BGA 补充剂在体内的安全性，也没有使用动脉粥样硬化动物模型来机械评估其动脉粥样硬化保护功效。此外，这是第一项将新培养的 NO 与目前作为膳食补充剂食用的培养 SP 进行比较的生物学功能的研究。 BGA 的培养可以规避 BGA 有争议的健康问题，因为在生产过程中可以严格控制质量和毒素污染。在这方面，培养的 NO 和 SP 比自然收获的 BGA 物种具有研究生物学功能的价值。就预期结果而言，具体目标 1-3 中提出的集体工作预计将通过阐明分子机制，深入了解 BGA 毒性和动脉粥样硬化保护特性。这些结果将为人体临床试验提供科学基础，以测试BGA作为有效且可靠的CAM物质降低CVD风险的功效，从而对公众健康产生重要的积极影响。因此，拟议的研究非常契合 NCCAM 的使命：“在严谨的科学背景下探索补充和替代治疗实践，培训 CAM 研究人员，并向公众和专业社区传播权威信息。” 公共健康相关性：蓝绿藻产品已作为膳食补充剂推出，以满足公众对使用天然产品预防和治疗心脏病的日益增长的兴趣。然而，各种 BGA 种类的安全性和生物功能尚未得到适当研究。该项目将评估新培育的蓝绿藻发菜品种的安全性和动脉粥样硬化保护特性。 sphaeroides Kutzing 与 Spirulina plantensis 一起，后者是当前膳食补充剂市场上最常见的培养 BGA。该项目涉及补充和替代医学 (CAM) 物质基础研究的一个重要但尚未得到充分研究的领域，该领域将为人体临床试验提供科学基础，以测试 BGA 作为有效和可靠的 CAM 物质的功效，从而对公众健康产生积极影响。以降低患心血管疾病的风险。

项目成果

期刊论文数量（8）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Blue-Green Algae Inhibit the Development of Atherosclerotic Lesions in Apolipoprotein E Knockout Mice.

蓝绿藻抑制载脂蛋白 E 敲除小鼠动脉粥样硬化病变的发展。

DOI：
发表时间：
2015-12
期刊：
影响因子：
2.4
作者：
Ku, Chai Siah;Kim, Bohkyung;Pham, Tho X;Yang, Yue;Wegner, Casey J;Park, Young;Balunas, Marcy;Lee, Ji
通讯作者：
Lee, Ji

Hypolipidemic Effect of a Blue-Green Alga (Nostoc commune) Is Attributed to Its Nonlipid Fraction by Decreasing Intestinal Cholesterol Absorption in C57BL/6J Mice.

蓝绿藻（发菜公社）的降血脂作用归因于其非脂质部分通过减少 C57BL/6J 小鼠肠道胆固醇吸收。

DOI：
发表时间：
2015-11
期刊：
影响因子：
2.4
作者：
Ku, Chai Siah;Kim, Bohkyung;Pham, Tho X;Yang, Yue;Weller, Curtis L;Carr, Timothy P;Park, Young;Lee, Ji
通讯作者：
Lee, Ji

In vitro and in vivo safety assessment of edible blue-green algae, Nostoc commune var. sphaeroides Kützing and Spirulina plantensis.

食用蓝绿藻（Nostoc commune var.）的体外和体内安全性评估。

DOI：
发表时间：
2011-07
期刊：
影响因子：
0
作者：
Yang, Yue;Park, Youngki;Cassada, David A.;Snow, Daniel D.;Rogers, Douglas G.;Lee, Jiyoung
通讯作者：
Lee, Jiyoung

Edible blue-green algae reduce the production of pro-inflammatory cytokines by inhibiting NF-κB pathway in macrophages and splenocytes.

食用蓝绿藻通过抑制巨噬细胞和脾细胞中的 NF-κB 通路来减少促炎细胞因子的产生。

DOI：
10.1016/j.bbagen.2013.01.018
发表时间：
2013-04
期刊：
Biochimica et biophysica acta
影响因子：
0
作者：
Ku, Chai Siah;Pham, Tho X.;Park, Youngki;Kim, Bohkyung;Shin, Min Sun;Kang, Insoo;Lee, Jiyoung
通讯作者：
Lee, Jiyoung

Health benefits of blue-green algae: prevention of cardiovascular disease and nonalcoholic fatty liver disease.

蓝绿藻的健康益处：预防心血管疾病和非酒精性脂肪肝。