Neuroendocrine Effects of Alcohol on Puberty

酒精对青春期神经内分泌的影响

• 批准号: 8266551
• 负责人: W L DEES
• 金额: $ 31.15万
• 依托单位: TEXAS A&M UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 2014-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8266551
• 关键词: Acute; Address; Adhesions; Adolescent; Alcohol consumption; Alcohols; Astrocytes; Brain; Cell Line; Chronic; Delayed Puberty; Development; Endocrine; Event; Female; Gene Proteins; Genes; Goals; Gonadotropin Hormone Releasing Hormone; Growth; Health; Hormonal; Hypothalamic Hormones; Hypothalamic structure; In Vitro; Molecular; Neurons; Neurosecretory Systems; Ovarian hormone; Peptides; Physiological; Pituitary Hormones; Process; Puberty; Rattus; Regulation; Research; Risk; Signal Transduction; Techniques; Testing; alcohol effect; alcohol exposure; in vivo; indexing

DESCRIPTION (provided by applicant): The increase in alcohol (ALC) use and abuse by adolescents during recent years poses several risks for them associated with growth, endocrine functions and development. The overall goal of this study is to obtain information which will allow us to better understand the detrimental effects and mechanisms of action of ALC on the female pubertal process. The studies proposed will assess influences of specific puberty related peptides, the involvement and activation of important genes critical to brain events leading to mammalian puberty, and discern the effects of ALC on their actions and interactions. In vivo and in vitro techniques using molecular and physiological approaches will be utilized following chronic and acute ALC exposure to address six aims which will collectively allow us to determine what factors are involved, the mechanisms of interference and potential means to reverse the detrimental effects of ALC on puberty related events. Aim 1 will assess the effects of ALC on the synthesis of specific puberty related hypothalamic genes and proteins, with the results being correlated with hypothalamic, pituitary and ovarian hormones, as well as other indices of puberty. Aim 2 will assess regulation of hypothalamic genes by peptides involved at puberty and determine the actions of ALC. Aim 3 will assess the expression and regulation of glial and neuronal signaling-adhesion genes at puberty and determine the effects of ALC. Aim 4 will assess the effects of ALC on the hypothalamic action of specific peptides that induce luteinizing hormone releasing hormone (LHRH) secretion at puberty. Aim 5 will use cultured rat hypothalamic astrocytes and an LHRH neuronal cell line to further assess glial-neuronal mechanisms associated with LHRH secretion, determine the actions of ALC, and investigate potential means for reversal of ALC actions. Aim 6 will test the ability of specific substances in vivo to protect against or reverse ALC-induced delayed puberty. This research, which is relevant to adolescent health and development, is intended to further identify consequences of ALC consumption with regard to hormonal events and their actions which can alter female pubertal maturation. Narrative The information gained from this study will allow us to better understand the detrimental effects and mechanisms of action of alcohol (ALC) on the female pubertal process. The studies proposed will assess influences of specific puberty related peptides, the involvement and activation of important genes critical to brain events leading to mammalian puberty, and discern the effects of ALC on their actions and interactions. This research, which is relevant to adolescent health and development, is intended to further identify consequences of ALC consumption with regard to hormonal events and their actions which can alter female pubertal maturation.

描述（由申请人提供）：近年来，青少年酒精（ALC）使用和滥用的增加给他们带来了与生长、内分泌功能和发育相关的多种风险。本研究的总体目标是获得信息，使我们能够更好地了解 ALC 对女性青春期过程的有害影响和作用机制。拟议的研究将评估特定青春期相关肽的影响、对导致哺乳动物青春期的大脑事件至关重要的重要基因的参与和激活，并辨别 ALC 对它们的作用和相互作用的影响。在慢性和急性 ALC 暴露后，将利用使用分子和生理学方法的体内和体外技术来实现六个目标，这将使我们能够共同确定涉及哪些因素、干扰机制以及逆转 ALC 有害影响的潜在方法与青春期相关的事件。目标 1 将评估 ALC 对特定青春期相关下丘脑基因和蛋白质合成的影响，其结果与下丘脑、垂体和卵巢激素以及其他青春期指标相关。目标 2 将评估青春期涉及的肽对下丘脑基因的调节，并确定 ALC 的作用。目标 3 将评估青春期神经胶质和神经元信号粘附基因的表达和调节，并确定 ALC 的效果。目标 4 将评估 ALC 对诱导青春期黄体生成素释放激素 (LHRH) 分泌的特定肽的下丘脑作用的影响。目标 5 将使用培养的大鼠下丘脑星形胶质细胞和 LHRH 神经元细胞系来进一步评估与 LHRH 分泌相关的神经胶质神经元机制，确定 ALC 的作用，并研究逆转 ALC 作用的潜在方法。目标 6 将测试体内特定物质预防或逆转 ALC 引起的青春期延迟的能力。这项与青少年健康和发展相关的研究旨在进一步确定食用 ALC 对荷尔蒙事件及其可能改变女性青春期成熟的作用的影响。叙述 从这项研究中获得的信息将使我们更好地了解有害的 酒精（ALC）对女性青春期过程的影响和作用机制。这 拟议的研究将评估特定青春期相关肽的影响， 参与和激活对大脑事件至关重要的重要基因，导致 哺乳动物青春期，并了解 ALC 对它们的行为和相互作用的影响。 这项与青少年健康和发展相关的研究旨在 进一步确定 ALC 消耗对激素事件的影响，以及 他们的行为可以改变女性青春期的成熟。