ANALYSIS OF RNA FROM ECTOPIC ENDOMETRIUM IN RHESUS MACAQUES

恒河猴异位子宫内膜 RNA 分析

• 批准号: 8357805
• 负责人: OV D SLAYDEN
• 金额: $ 4.36万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357805
• 关键词: Affinity; Androgen Antagonists; Androgen Receptor; Anti-Progestin; Archives; Bicalutamide; Biological Models; Cell Proliferation; Data; Endometrial; Endometrium; Estrogens; Funding; Gene Expression; Genes; Genome; Goals; Grant; Intra-abdominal; Macaca; Macaca mulatta; Mineralocorticoid Receptor; Modeling; Molecular Analysis; National Center for Research Resources; Preclinical Testing; Primates; Principal Investigator; Progesterone Receptors; RNA analysis; Research; Research Infrastructure; Resources; Sampling; Selective Estrogen Receptor Modulators; Site; Source; Spironolactone; Techniques; Testing; Tissue-Specific Gene Expression; Transplantation; United States National Institutes of Health; cost; endometriosis; eplerenone; novel; subcutaneous

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Macaque endometrium can be auto-transplanted to ectopic intra-abdominal and subcutaneous sites. These endometrial grafts provide an excellent endometriosis-like model system for preclinical testing of novel anti-endometriotic therapies. In this model we have tested the effects of anti-estrogenic selective estrogen receptor modulators (SERMs), spironolactone (anti-mineralocorticoid receptor, anti-MR; anti-progesterone receptor, anti-PR; anti-androgen receptor, anti-AR) with Eplerenone (an anti-MR with no known affinity for AR), Casodex, (a pure anti-AR) and ZK 099 (a pure anti-PR) on estrogen action on ectopic endometrium in rhesus macaques. These samples were analyzed by morphological and immunocytochemical techniques for cell proliferation. The remaining samples arre archived for molecular analysis. Our goal is to analyze gene expression in these archived samples, using the Affymetrix GeneChip¿ Rhesus Macaque Genome Array. The resulting data on differential gene expression will be used to identify genes differentially regulated by these proprietary compounds.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 可以将猕猴子宫内膜自动转移至异位腹内和皮下部位。这些子宫内膜移植物为新型抗内膜疗法的临床前测试提供了出色的子宫内膜异位症模型系统。在该模型中，我们测试了抗雌激素选择性雌激素受体调节剂（SERMS），螺内乳酮（抗矿物皮质激素受体，抗MR;抗MR;抗孕酮受体，抗PR;抗PR;抗抗激素受体，抗AR，抗AR）抗epleronone（抗Anti）（抗抗苯乙烯的抗）（无知的均为AR）和CASKKEN（AR），AR），AR CASSKAR，A.A.A.A.A（A）纯抗PR）对猕猴中雌激素作用对依托po子宫内膜的作用。通过形态和免疫细胞化学技术分析这些样品，以进行细胞增殖。其余样品存档用于分子分析。我们的目标是使用AffymetrixGenechip¿Rhesus猕猴基因组阵列分析这些存档样品中的基因表达。最终的差异基因表达数据将用于识别这些专有化合物对不同调节的基因。