PRIMATE MODEL FOR MYCOPLASMA GENITALIUM

生殖支原体灵长类动物模型

• 批准号: 8357617
• 负责人: PATRICIA A TOTTEN
• 金额: $ 15.66万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357617
• 关键词: Animal Model; Animals; Antibodies; Antibody Formation; Bacterial Adhesins; Biopsy; Cells; Cervical; Cervix Uteri; Detection; Funding; Grant; Histology; Immune; Infection; Infiltration; Macaca nemestrina; Mammalian Oviducts; Modeling; Mycoplasma genitalium; National Center for Research Resources; Primates; Principal Investigator; Process; Production; Protocols documentation; Research; Research Infrastructure; Resources; Schedule; Serum; Source; Specimen; Time; Tissues; United States National Institutes of Health; Vagina; Variant; cost; fimbria; pathogen; reproductive; research study; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In our grant, IR21AI074898, "A Primate Model of Mycoplasma genitalium" we are assessing the ability of pigtail macaques to serve as an animal model to study the immunopathogenesis of M. genitalium (MG), a newly recognized reproductive tract pathogen. In this model primates are inoculated cervically with MG then vaginal and cervical specimens are collected at intervals spanning 8 weeks for detection of MG by qPCR and culture. Biopsies are collected at Week 4 (Fallopian tube and fimbriae) and Week 8 (Fallopian tube, fimbriae and cervix) at which time the animals are treated to cure the MG infection. The biopsies are processed for culture, qPCR and histology to detect immune cell infiltration. Serum is collected prior to infection at and intervals to assess the production of anti-MG antibodies over the course of the infection. In the past year we have inoculated 4 of the 6 primates funded by this proposal with MG: 2 primates were successfully colonized throughout the 8 weeks of the study, one was colonized for 4-6 weeks and the fourth animal was not infected. Preliminary analysis of serum suggests that antibodies to MG are produced after infection that specifically recognized the immunodominant adhesin molecules, MgpB and MgpC. Culture and qPCR of the upper reproductive tract tissues suggests that MG has not ascended from the lower tract in these animals suggesting that longer experiments or repeated inoculations may be necessary to induce upper tract infection. Finally, experiments are underway to assess sequence variation in MgpB and MgpC during infection in response to antibody production. The final two primates funded by this grant are scheduled to undergo our protocol, scheduled for Feb-Aug 2011.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 在我们的IR21AI074898的赠款中，“是支原体生殖器的灵长类动物模型”，我们正在评估尾尾猕猴作为动物模型研究的能力，以研究一种新认识的生殖器生殖器（MG）的免疫病作用，这是一种新认识的生殖牵引病原体。 在此模型中，灵长类动物与MG接种接种，然后在QPCR和培养物检测8周的时间间隔内进行阴道和宫颈标本。 活检是在第4周（输卵管和叶片）和第8周收集活检（输卵管，fimbriae和宫颈），在此期间，对动物进行治疗以治愈MG感染。 进行活检以用于培养，QPCR和组织学以检测免疫细胞浸润。 在感染过程中，在感染之前和间隔收集血清，以评估在感染过程中的抗MG抗体的产生。 在过去的一年中，我们已经接种了由Mg：2个提案资助的6个灵长类动物中的4个：2个灵长类动物在整个研究的整个研究中成功殖民，其中一名被殖民4-6周，而第四只动物未被感染。 血清的初步分析表明，感染后产生了与MG的抗体，该抗体是特异性识别免疫主导粘附素分子MGPB和MGPC的。上部生殖道组织的培养和QPCR表明，在这些动物中，MG并未从下层中升起，这表明可能需要更长的实验或重复接种以诱导上层裂纹感染。最后，正在进行实验，以评估响应抗体产生过程中感染过程中MGPB和MGPC的序列变化。 这笔赠款资助的最后两位灵长类动物计划在2011年2月至8月进行我们的协议。