GENE-TARGETTED SNP DISCOVERY IN RHESUS MACAQUES

恒河猴中基因靶向 SNP 的发现

• 批准号: 8357783
• 负责人: BETSY M FERGUSON
• 金额: $ 5.82万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357783
• 关键词: 3' Untranslated Regions; Chinese People; Data; Databases; Funding; Gene Expression Profile; Gene Frequency; Gene Targeting; Genes; Grant; Macaca mulatta; Messenger RNA; National Center for Research Resources; Population; Prefrontal Cortex; Primates; Principal Investigator; Reading; Reporting; Research; Research Infrastructure; Resources; Single Nucleotide Polymorphism; Source; Technology; United States National Institutes of Health; cost; next generation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This project originally aimed to identify single nucleotide polymorphisms (SNPs) in 10,000 gene regions, by sequencing the 3' UTR of unrelated Chinese and Indian rhesus macaques. The compiled SNP data will be analyzed to identify SNPs and allele frequencies in both Indian and Chinese populations and to report the findings in the public databases dbSNP and MonkeySNP. In the past year we adjusted our SNP discovery strategy to include next generation sequencing technologies (Illumina short-read formats). We completed transcriptome sequencing using mRNA from the prefrontal cortex of six Indian and six Chinese rhesus macaques. Sanger re-sequencing was used to validate the candidate SNPs and to inform filtering limits to insure robust data. Over 1 million SNPs were detected in this study thus far.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 该项目最初旨在通过测序无关的中国和印度恒河猕猴的3'UTR来确定10,000个基因区域中的单核苷酸多态性（SNP）。将分析编制的SNP数据，以识别印度和中国人群中的SNP和等位基因频率，并在公共数据库DBSNP和Monkeysnp中报告结果。在过去的一年中，我们调整了SNP发现策略，以包括下一代测序技术（Illumina短阅读格式）。 我们使用来自六个印度和六个中国恒河猕猴的前额叶皮层的mRNA完成了转录组测序。 Sanger重新测序用于验证候选SNP并告知过滤限制以确保鲁棒数据。到目前为止，这项研究中检测到了超过100万个SNP。