Identification of Tissue Specific Stem Cells

组织特异性干细胞的鉴定

• 批准号: 8212936
• 负责人: SAUL Joseph SHARKIS
• 金额: $ 30.3万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-02-01 至 2013-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8212936
• 关键词: Adult; Angiopoietins; Animal Model; Belief; Biological; Biological Assay; Biology; Bone Marrow; Bone Marrow Transplantation; Cell Separation; Cells; Characteristics; Clinical Trials; Cytokine Signaling; Data; Daughter; Embryo; Engraftment; Epithelial; Experimental Designs; Failure; Germ Layers; Goals; Hematopoietic Stem Cell Transplantation; In Vitro; Injury; LIF gene; Location; Lung; Malignant Neoplasms; Marrow; Measures; Methods; Mus; Organ; Pancreas; Physiology; Pluripotent Stem Cells; Population; Principal Investigator; Proliferating; Property; Proteins; Publishing; Relative (related person); Side; Signal Pathway; Signal Transduction; Site; Stem cells; Surface Antigens; Target Populations; Technology; Testing; Time; Tissues; Transplantation; adult stem cell; base; cancer stem cell; cell type; design; embryonic stem cell; hematopoietic tissue; human disease; human embryonic stem cell; improved; in vivo; injured; injury and repair; insight; novel; programs; regenerative; repaired; research study; response to injury; self-renewal; stem cell biology; stem cell fate; stem cell population; success; therapeutic target

Our Specific aims continue to explore the biology of stem cells. Specific aim 1 uses simplified gradient separation to isolate stem cells. We plan studies which will isolate stem cells by functional characteristics. We believe that the most primitive quiescent stem cell may not express cell surface antigens attributable to long term engrafting cells. We have evidence that mul tipotential stem cells can proliferate and differentiate into cells of different germ layers. We plan additional clonal a nalysis to evaluate the engraftment capacity of pure stem cell populations. We will examine the nonhematopoieitc (epithelial) engraftment of our enriched populations of marrow derived stem cells. We plan to examine the factors which are known to regulate the proliferation and differentiation of mouse adult and embryonic stem cell populations. Finally, in specific aim 1, we will determine if stem cells isolated by gradient separation technology have the capacity to convert marrow cells into epithelial tissues in response to injury signals. In the second aim, importantly, we will attempt to distinguish between the presence of tissue specific stem cells and the existence of a pluripotent stem cell which resides in the bone marrow which, when stimulated by external injury signals, will respond by mobilization to the site of injury, convert into a new germ layer, and aid in repair of the injury. Relevance Bone marrow transplantation has been utilized for hematological failure or malignancy with increasing success. Recent animal models and a few clinical trials have used stem cell populations to repair injury via transplantation. We are attempting to define the cell types which can be used for such repair, develop simple and universal methods for isolating those cells and determining what factors in the microenvironment are responsible for stem cell self renewal and differentiation.

我们的具体目的继续探索干细胞的生物学。特定目标1使用简化梯度 分离以分离干细胞。我们计划将通过功能特征分离干细胞的研究。 我们认为，最原始的静态干细胞可能无法表达细胞表面抗原 长期雕刻细胞。我们有证据表明MUL尖端干细胞可以增殖和区分 进入不同细菌层的细胞。我们计划其他克隆分析，以评估 纯干细胞种群。我们将研究我们丰富的非杂型（上皮）植入 骨髓衍生的干细胞种群。我们计划检查已知的因素 小鼠成人和胚胎干细胞种群的增殖和分化。最后，以特定的目的 1，我们将确定通过梯度分离技术隔离的干细胞是否具有转换的能力 骨髓细胞响应损伤信号而进入上皮组织。在第二个目标中，重要的是，我们将 试图区分组织特异性干细胞的存在和多能的存在 驻留在骨髓中的干细胞，当外部受伤信号刺激时，将通过 动员受伤部位，转化为新的生殖层，并有助于修复伤害。 关联 骨髓移植已用于血液学衰竭或恶性肿瘤随着增加而增加 成功。最近的动物模型和一些临床试验已使用干细胞群来修复损伤 移植。我们正在尝试定义可用于维修的单元格类型，开发简单 和隔离这些细胞并确定微环境中哪些因素的通用方法是 负责干细胞自我更新和分化。