Histopathology

组织病理学

• 批准号: 8320336
• 负责人: PELAYO none CORREA
• 金额: $ 12.81万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8320336
• 关键词: 3-nitrotyrosine; Biopsy; Buffers; Caring; Colombia; DNA; Disease; Evaluation; Formalin; Freezing; Frozen Sections; Gastroscopes; Helicobacter pylori; Hematoxylin and Eosin Staining Method; Histology; Histopathology; Human; Human Resources; Ice; Infection; Laboratories; Leukocytes; Methylation; Microdissection; Microscope; Mucous Membrane; Paraffin; Paraffin Embedding; Procedures; Process; Research Personnel; Rodent; Silver Staining; Staining method; Stains; Stomach; Stomach Carcinoma; TdT-Mediated dUTP Nick End Labeling Assay; Tissues; Training; Zinc; experience; human NOS2A protein; tissue processing; 3-nitrotyrosine; Biopsy; Buffers; Caring; Colombia; DNA; Disease; Evaluation; Formalin; Freezing; Frozen Sections; Gastroscopes; Helicobacter pylori; Hematoxylin and Eosin Staining Method; Histology; Histopathology; Human; Human Resources; Ice; Infection; Laboratories; Leukocytes; Methylation; Microdissection; Microscope; Mucous Membrane; Paraffin; Paraffin Embedding; Procedures; Process; Research Personnel; Rodent; Silver Staining; Staining method; Stains; Stomach; Stomach Carcinoma; TdT-Mediated dUTP Nick End Labeling Assay; Tissues; Training; Zinc; experience; human NOS2A protein; tissue processing

Core A takes care of the tissue processing needs for all projects. It is directly supervised by Dr. Pelayo Correa and his assistant Dr. M. Blanca Piazuelo. In Nashville our personnel receive all biopsies from Colombia. Biopsies for histopathology evaluation are fixed immediately in buffered zinc-formalin, embedded in paraffin, sectioned and stained with hematoxylin eosin and modified Warthin- Starry silver stain. Biopsies for frozen sections are received in OCT and frozen immediately. The laboratory performs all immunohistochemical stains, including Ki67, TUNEL assays, inducible nitric oxide synthase, nitrotyrosine and a variety of white blood cell markers. Fully trained and experienced personnel are available for all procedures.

核心A负责所有项目的组织处理需求。它是由Pelayo博士直接监督的 科雷亚和他的助手M. Blanca Piazuelo博士。在纳什维尔，我们的人员从 哥伦比亚。用于组织病理学评估的活检立即固定在缓冲锌 - 法拉林中，嵌入 在石蜡中，用苏木精植物截切和染色，并改良的warthin-arthin-arthry银染色。活检 对于冷冻部分，在10月收到并立即冷冻。实验室执行所有操作 免疫组织化学染色，包括KI67，TUNEL分析，诱导型一氧化氮合酶，硝基酪氨酸 以及各种白细胞标记。全面训练和经验丰富的人员可供所有人使用 程序。