Leica SP5 confocal microscope for live-cell imaging

用于活细胞成像的 Leica SP5 共焦显微镜

• 批准号: 8247512
• 负责人: COLIN J BARNSTABLE
• 金额: $ 58.7万
• 依托单位: PENNSYLVANIA STATE UNIV HERSHEY MED CTR
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-06-01 至 2013-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8247512
• 关键词: Alzheimer' s Disease; Cells; Core Facility; Degenerative Disorder; Detection; Diabetes Mellitus; Diabetic Retinopathy; Dyes; Equipment; Event; Eye diseases; Fluorescence; Funding; Glaucoma; Health; Heart Diseases; Image; Ions; Label; Life; Medicine; Metabolic; Microscope; Mitochondria; Muscle Proteins; Parkinson Disease; Peripheral Nervous System; Photobleaching; Protein Biosynthesis; Proteins; Recovery; Research Design; Research Personnel; Resolution; Restless Legs Syndrome; Scanning; Speed; Time; Tissues; United States National Institutes of Health; Virus Diseases; cellular imaging; college; fluorophore; genetic manipulation; improved; instrument; meetings; protein expression; research study; response; trafficking; transmission process

DESCRIPTION (provided by applicant): The objective of this application is to purchase a new confocal microscope for the Penn State Hershey College of Medicine Core Imaging Facility. Though our core facilities are available to all investigators in the College of Medicine, this request is submitted to meet the specific needs of a group of NIH-funded major users. This new microscope will permit three types of study that cannot be accomplished with our current facility. First, because the microscope will be configured specifically for live-cell studies, we can meet the rapidly growing need for more sophisticated equipment dedicated to this experimental approach. The configuration will enable precise execution of time lapse studies, fluorescence resonance energy transmission (FRET) and fluorescent recovery after photobleaching (FRAP). Second, the microscope will also have a tandem scanner to provide both high resolution and high speed scanning options. These features will be vital for high resolution imaging experiments and will allow dynamic studies of ion flow and mitochondrial status. Third, the instrument will allow the simultaneous excitation and detection of up to five different fluorophores. The availability of this new microscope will improve the ability to perform intensive live-cell imaging projects focusing on a variety of health issues including viral infections, cance, diabetes and its complications (primarily diabetic retinopathy and muscle protein synthesis), heart disease, degenerative diseases of the central and peripheral nervous system (including Parkinson's disease, Alzheimer's disease, restless leg syndrome and eye diseases such as glaucoma). The projects will use the equipment to image rapid events in live cells or explant tissue transfected with fluorescent protein markers, or labeled with metabolic dyes, to determine changes in protein expression, trafficking and redistribution in response to pharmacological, environmental or genetic manipulations. PUBLIC HEALTH RELEVANCE: The confocal microscope requested will have broad relevance to human health because it will enable us to study rapid interactions between molecules in living cells, in real time and thus aid determination of their function in health and disease. This instrument will enhance a wide variety of public health projects being conducted at the Milton S. Hershey Medical Center, including those related to cancer, diabetes, heart disease, viral infection and neurodegenerative diseases.

描述（由申请人提供）：本申请的目的是为Penn State Hershey医学院核心成像设施购买新的共聚焦显微镜。尽管我们的核心设施可用于医学院的所有调查人员，但提出了该请求以满足一组NIH资助的主要用户的特定需求。这种新的显微镜将允许我们当前的设施无法完成三种类型的研究。首先，由于显微镜将专门为实时研究配置，因此我们可以满足对这种实验方法的更复杂设备的快速增长的需求。该配置将使时间流逝研究，荧光共振能量传播（FRET）和光漂白后的荧光回收率（FRAP）精确执行。其次，显微镜还将具有串联扫描仪，以提供高分辨率和高速扫描选项。这些特征对于高分辨率成像实验至关重要，并且将允许对离子流和线粒体状态的动态研究。第三，该仪器将同时激发和检测多达五个不同的荧光团。该新显微镜的可用性将提高执行密集型的能力 活细胞成像项目的重点是各种健康问题，包括病毒感染，CANCE，糖尿病及其并发症（主要是糖尿病性视网膜病和肌肉蛋白质合成），心脏病，中枢和外周神经系统的退化性疾病（包括帕金森氏病，包括帕金森氏病，阿尔茨海默氏病，阿尔茨海默氏病，不休息的腿部和眼部疾病）。这些项目将使用设备来对活细胞中的快速事件或用荧光蛋白标记转染或用代谢染料标记的外植体组织，以确定蛋白质表达，运输和重新分布的变化，以响应药理，环境或遗传操作。 公共卫生相关性：所需的共聚焦显微镜将与人类健康具有广泛的相关性，因为它将使我们能够实时研究活细胞中分子之间的快速相互作用，从而有助于确定其在健康和疾病中的功能。该工具将增强在米尔顿·赫尔希医学中心（Milton S. Hershey Medical Center）进行的各种公共卫生项目，包括与癌症，糖尿病，心脏病，病毒感染和神经退行性疾病有关的公共卫生项目。

项目成果

An autophagy assay reveals the ESCRT-III component CHMP2A as a regulator of phagophore closure.

• DOI: 10.1038/s41467-018-05254-w
• 发表时间: 2018-07-20
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Takahashi Y;He H;Tang Z;Hattori T;Liu Y;Young MM;Serfass JM;Chen L;Gebru M;Chen C;Wills CA;Atkinson JM;Chen H;Abraham T;Wang HG
• 通讯作者: Wang HG

A Nanomule Peptide Carrier Delivers siRNA Across the Intact Blood-Brain Barrier to Attenuate Ischemic Stroke.

• DOI: 10.3389/fmolb.2021.611367
• 发表时间: 2021
• 期刊: Frontiers in molecular biosciences
• 影响因子: 5
• 作者: Eyford BA;Singh CSB;Abraham T;Munro L;Choi KB;Hill T;Hildebrandt R;Welch I;Vitalis TZ;Gabathuler R;Gordon JA;Adomat H;Guns EST;Lu CJ;Pfeifer CG;Tian MM;Jefferies WA
• 通讯作者: Jefferies WA