Tissue Management Shared Resource

组织管理共享资源

• 批准号: 8305125
• 负责人: JAMES K. WILLSON
• 金额: $ 9.37万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8305125
• 关键词: African American; Anatomy; Biocompatible Materials; Blood; Bone Marrow; Breast; Cancer Center Support Grant; Caucasians; Caucasoid Race; Cells; Clinical; Colon; Computer software; Consent; DNA; Data; Databases; Ensure; Female; Freezing; Growth; Hispanics; Histology; Human; Individual; Institutional Review Boards; Kidney; Laboratories; Lasers; Liquid substance; Lung; Malignant Neoplasms; Medical Records; Operative Surgical Procedures; Ovary; Paraffin; Participant; Pathologist; Pathology; Patient Participation; Prostate; Quality Control; RNA; Research; Resource Sharing; Resources; Sampling; Secure; Services; Specimen; Staining method; Stains; Tissue Procurements; Tissues; base; demographics; tissue culture; web interface

The Tissue Management Shared Resource provides IRB-approved centralized tissue procurement services for various specimens derived from consented human participants. Over 4500 tissue specimens and bodily fluids, from 52 different fissue types, especially blood, bone marrow, breast, colon, kidney, lung, ovary and prostate, and 84 defined cancer subtypes are available. In terms of demographics, 65%, 17% and 15% of specimens are derived from Caucasian, African-American and Hispanic participants, respectively; 59% of specimens are derived from female participants. All fissue specimens are evaluated by a board-certified anatomic pathologist for quality control purposes, ensuring that the specimens used for research are of the highest quality. For every specimen, extensive data is captured from the participant's medical record, including demographics, clinical findings, diagnosfic laboratory tesfing results, treatment information and pathology assessment results. These data are captured and stored in the UT Southwestern Biomaterial Tracking and Management (BTM) software application, which includes a relafional database that stores these data in a de-identified manner that can be accessed and searched through a secure web interface to authorized invesfigators. In addifion to procurement services, the resource offers a number of other ancillary services. DNA and RNA fractions are routinely isolated from procured specimens. Standard histology services - embedding (fresh and fixed), secfioning (frozen and fixed), and staining - are offered for all specimens. Laser capture microdissecfion of fissue secfions is offered for invesfigators wishing to isolate specific cells within a fissue sample for further analysis. Laser capture is offered at two levels of resolufion - capture of fissue regions and capture of individual cell sets - depending on the specific research needs of the invesfigator. The resource offers services for the construcfion of fissue microarray in which as many as 768 samples can be arrayed in a single paraffin block and sectioned for immunohistochemical analysis. The resource is equipped for the isolafion and growth of primary cells from pafient samples for tissue culture studies and xenotransplantafion. In order to ensure the highest level of compliance and consistency, resource staff routinely takes responsibility for consenting surgical patients for participation in fissue procurement-based research.

组织管理共享资源提供 IRB 批准的集中组织采购服务 对于来自同意的人类参与者的各种标本。超过 4500 个组织样本和身体样本 来自 52 种不同组织类型的液体，特别是血液、骨髓、乳腺、结肠、肾、肺、卵巢和 前列腺癌和 84 种明确的癌症亚型可用。从人口结构来看，分别为 65%、17% 和 15% 样本分别来自白种人、非裔美国人和西班牙裔参与者； 59%的 标本来自女性参与者。所有组织样本均由经过委员会认证的专家进行评估 解剖病理学家出于质量控制目的，确保用于研究的标本属于 最高品质。对于每个样本，都会从参与者的医疗记录中捕获大量数据， 包括人口统计、临床结果、诊断实验室测试结果、治疗信息和 病理评估结果。这些数据被捕获并存储在 UT Southwestern Biomaterial 中 跟踪和管理 (BTM) 软件应用程序，其中包括一个存储 这些数据以去识别化的方式进行，可以通过安全的网络界面访问和搜索 授权调查员。除了采购服务外，该资源还提供许多其他辅助服务 服务。 DNA 和 RNA 组分通常从采购的样本中分离出来。标准组织学 为所有人提供包埋（新鲜和固定）、切片（冷冻和固定）和染色服务 标本。为希望分离的研究人员提供组织切片的激光捕获显微切割 组织样本中的特定细胞用于进一步分析。激光捕获提供两种分辨率级别 - 捕获组织区域和捕获单个细胞组 - 取决于具体研究需求 调查员。该资源为组织微阵列的构建提供服务，其中多达 768 个样品可以排列在单个石蜡块中并切片用于免疫组织化学分析。这 配备有用于从患者样本中分离和生长原代细胞以进行组织培养的资源 研究和异种移植。为了确保最高水平的合规性和一致性， 资源工作人员通常负责同意手术患者参与组织 基于采购的研究。