Facility Core B: Molecular Biology

设施核心 B：分子生物学

• 批准号: 8250000
• 负责人: Jianjun Shen
• 金额: $ 25.28万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2013-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8250000
• 关键词: Biological Assay; Computer software; Consultations; Core Facility; Data Analyses; Databases; Disease; Equipment; Gene Expression; Gene Proteins; Goals; Image; Instruction; Laboratories; Molecular; Molecular Biology; Nature; Preparation; Prevention; Procedures; Protein Analysis; Research Personnel; Robotics; Sampling; Sequence Analysis; Services; Techniques; Training; comparative; digital imaging; instrument; instrumentation; meetings; member; multidisciplinary; new technology; protein expression; response

Overall Objectives and Specific Aims The overall goal of the Molecular Biology Facility Core is to provide Center Investigators access to sophisticated and state-of-the-art molecular techniques, services, and instrumentation. Because the Center is multidisciplinary in nature, the level of support needed by different Center investigators varies widely. Consequently, the level of access offered by the Molecular Biology Facility Core varies from consultation and advice on specific aspects of projects, through training on particular techniques and/or instruments available in the Core, to complete molecular analyses from sample preparation to the final data analysis and interpretation. The Specific Aims of Facility Core 1 are: 1. to continue to provide molecular biology services and assays in response to the needs of Center Members, including a variety of DMA level techniques, comparative expression analysis and protein separation, identification and analysis; 2. to provide instruction and assistance to Center investigator laboratories in the use of macroscopic image equipment and computer software for digital image acquisition and analysis, sequence data analysis, and sequence database searching; 3. to provide supervised access to realtime PCR, high throughput sequencing, fragment analysis, and robotic equipment; 4. to provide expertise, consultation and training for a wide range of molecular biology procedures, especially in gene expression and protein expression techniques; 5. to identify, introduce/develop, and implement new technologies and new assays to meet the needs of Center Members and the emerging needs of new Center initiatives.

总体目标和特定目标 分子生物学设施核心的总体目标是为中心调查人员访问 精致，最先进的分子技术，服务和仪器。因为中心是 本质上，多学科，不同中心研究人员所需的支持水平差异很大。 因此，分子生物学设施核心提供的访问水平随咨询和 有关项目的特定方面的建议，通过有关特定技术和/或工具的培训 核心，从样本制备到最终数据分析和解释的分子分析。 设施核心1的具体目的是： 1。继续提供分子生物学服务和针对中心需求的测定 成员，包括各种DMA水平技术，比较表达分析和蛋白质 分离，识别和分析； 2。为中心研究人员实验室提供指导和帮助，以使用宏观 图像设备和计算机软件用于数字图像获取和分析，序列数据 分析和序列数据库搜索； 3。为了提供对实时PCR的监督访问，高吞吐量测序，碎片分析和 机器人设备； 4。为广泛的分子生物学程序提供专业知识，咨询和培训， 特别是在基因表达和蛋白质表达技术中； 5。识别，介绍/开发并实施新技术和新测定，以满足需求 中心成员以及新中心计划的新兴需求。