ISOLATION AND IDENTIFICATION OF TWO NOVEL SDS-RESISTANT SECRETED CHITINASES

两种新型抗SDS分泌型几丁质酶的分离与鉴定

• 批准号: 7954007
• 负责人: Chang Liu
• 金额: $ 0.28万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954007
• 关键词: 2-Mercaptoethanol; Aeromonas; Chitin; Chitinase; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Institution; Mass Spectrum Analysis; Measurement; Molecular Weight; N-terminal; Oligosaccharides; Proteins; Research; Research Personnel; Resistance; Resources; Soil; Source; Taiwan; United States National Institutes of Health; biomedical resource; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have isolated two SDS-resistant endochitinases, designated as ASCHI53 and ASCHI61, from Aeromonas schubertii in the soil of southern Taiwan. Mass measurement with MALDI-TOF indicates that the molecular weights are 53,527 Da for ASCHI53 and 61,202 Da for ASCHI61. N-terminal sequencing of these two chitinases suggests that thye are novel proteins. Degredation of chitin with these two endochitinases gives rise to hexameric chitin oligosaccharide, a compound known to have various potent biomedical functions. In the presence of 5% SDS, ASCHI53 and ASCHI61 retain 65% and 75% chitinase activities, respectively while the chitinolytic activities completely retain even in the presence of 10% beta-mercaptoethanol.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们从台湾南部土壤中的舒伯特气​​单胞菌中分离出两种 SDS 抗性内切几丁质酶，命名为 ASCHI53 和 ASCHI61。 MALDI-TOF 质量测量表明 ASCHI53 的分子量为 53,527 Da，ASCHI61 的分子量为 61,202 Da。这两种几丁质酶的 N 端测序表明它们是新型蛋白质。 这两种内切几丁质酶降解几丁质产生六聚几丁质寡糖，这是一种已知具有多种有效生物医学功能的化合物。 在 5% SDS 存在下，ASCHI53 和 ASCHI61 分别保留 65% 和 75% 几丁质酶活性，而即使在 10% β-巯基乙醇存在下，几丁质分解活性也完全保留。