Innovative Biofabrication of 3D Nano-Biocomposites for Repair of Osteochondral De

用于修复骨软骨病的 3D 纳米生物复合材料的创新生物制造

基本信息

批准号：
8299911
负责人：
Laurie O'Rourke
金额：
$ 14.96万
依托单位：
BC GENESIS, LLC
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-09-01 至 2013-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8299911
关键词：
Age Air Architecture Area Bacteria Biocompatible Materials Biomechanics Bioreactors Blood Bone Regeneration Calcium ion Cartilage Cells Cellulose Chemistry Collagen Congenital Abnormality Defect Degenerative polyarthritis Deposition Disasters Disease Gluconacetobacter xylinus Hospitals Hydrogels Hydroxyapatites Implant In Situ Legal patent Mechanics Medical Methods Morphology Motor Operative Surgical Procedures Organ Organ Transplantation Orthopedics Outcome Patients Porosity Procedures Process Production Property Regenerative Medicine Research Site Solvents Stem cells Surface Surface Properties System Technology Testing Transplanted tissue Trauma Weight-Bearing state articular cartilage biomaterial compatibility bone cold temperature cost design design and construction electric field implantable device improved functioning innovation joint loading manufacturing process nano nanofiber nanomaterials novel osteochondral repair osteochondral tissue osteogenic reconstruction repaired scaffold sugar

项目摘要

DESCRIPTION (provided by applicant): The research presented in this proposal aims to solve one of the major limitations in implant repair of osteochondral defects, namely the control of architecture and morphology of replacement biomaterials. Osteochondral defects have an extremely limited potential for self-repair. Whether the defect is traumatic or degenerative, osteoarthritis frequently results from these defects necessitating surgical repair. Surgical treatment of large osteochondral defects is often accomplished by transferring a non-weight bearing section of bone and cartilage to the defect. This is a costly and invasive procedure without a reliable outcome for patients. An attractive alternative is to replace the defect with a single material that restores lost bone and simultaneously replaces the lost cartilage with material to cushion the compressive, tensile, and shearing forces of joint loading. The innovation in this proposal is novel biofabrication process for creating 3D Nano- cellulose hydroxyapatite biocomposite (Nano-biocomposite) which functions as a load bearing articular cartilage and its underlying bone for repair of large osteochondral defects. Our manufacturing process is capable of producing this unique biomaterial with gradient of properties at large scale, at low cost and with great environmental efficiency. Bacterial cellulose, BC is an emerging nano-biomaterial consisting of cellulose nanofibril networks produced by bacteria Acetobacter xylinum. It is a hydrogel-like biomaterial with unique biocompatibility, mechanical integrity, hydroexpansivity, and stability under a wide range of conditions. The similarity of size of cellulose nanofibrils with collagen makes cellulose an ideal scaffolding material for regenerative medicine. We propose to develop a biofabrication process of gradient 3D Nano- biocomposites for repair of osteochondral defects. We have made innovations with which we can manufacture bacterial cellulose nano-biomaterial with spatially controlled architecture and surface properties. PUBLIC HEALTH RELEVANCE: Reconstruction of orthopedic defects that arise from trauma, disease, age, or congenital defects is a necessary procedure to protect vital organs, restore motor function, and improve patient self-confidence. In the US an estimated 800,000 grafting procedures were performed in 2003 making bone the second most transplanted tissue after blood. Osteochondral defects are among large unmet medical needs. The research presented in this proposal aims to solve one of the major limitations in implant repair of osteochondral defects, namely the control of architecture and morphology of replacement biomaterials.

描述（由申请人提供）：本提案中提出的研究旨在解决植入骨软骨缺陷的主要局限性之一，即对建筑的控制和替代生物材料的形态的控制。骨软骨缺陷的自我修复潜力极为有限。无论缺陷是创伤性的还是退化性的，骨关节炎都经常是由于需要进行手术修复的这些缺陷而导致的。大骨软骨缺损的手术治疗通常是通过将骨骼和软骨的非重量轴承段转移到缺陷中来完成的。这是一种昂贵且侵入性的程序，没有患者的可靠结果。一个有吸引力的替代方法是用单个材料代替缺陷，该材料恢复损失的骨骼，并同时用材料代替损失的软骨，以缓冲关节载荷的压缩，拉伸和剪切力。该提案中的创新是新的生物制造过程，用于创建3D纳米 - 纤维素羟基磷灰石生物复合材料（纳米生物复合材料），该过程可作为负载的关节软骨及其基础骨骼的负载，用于修复大骨软骨缺陷。我们的制造工艺能够以低成本和良好的环境效率以大规模的大规模梯度生产这种独特的生物材料。 BC细菌纤维素是一种新兴的纳米生物材料，由细菌乙酰杆菌木质细菌产生的纤维素纳米纤维网络组成。它是一种像水凝胶一样的生物材料，具有独特的生物相容性，机械完整性，水力传播和稳定性在广泛的条件下。大小的相似性用胶原蛋白的纤维素纳米纤维的含量使纤维素成为再生医学的理想脚手架材料。我们建议开发梯度3D纳米生物复合材料的生物制造过程，以修复骨软骨缺损。我们进行了创新，可以通过空间控制的结构和表面特性来生产细菌纤维素纳米生物材料。公共卫生相关性：由创伤，疾病，年龄或先天性缺陷引起的骨科缺陷的重建是保护重要器官，恢复运动功能并改善患者自信心的必要程序。在美国，估计进行了800,000个嫁接程序，使骨骼成为仅次于血液的第二大移植组织。骨软骨缺陷是巨大的未满足的医疗需求。该提案中提出的研究旨在解决植入骨软骨缺陷的植入物修复的主要局限性之一，即对建筑的控制和替代生物材料的形态。