Assay Dev/Adapt (California)

检测开发/适应（加利福尼亚州）

• 批准号: 8120934
• 负责人: Steven J Brown
• 金额: $ 294.99万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2014-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8120934
• 关键词: Adherence; Applications Grants; Automation; Biological Assay; Business Rules; Calcium; California; Cataloging; Catalogs; Cell Count; Cell Line; Cells; Chemicals; Chemistry; Collaborations; Color; Computer software; Critical Pathways; Cyclic AMP; Data; Data Files; Data Quality; Databases; Detection; Development; Development Plans; Dimethyl Sulfoxide; Dose; Ensure; Enzymes; Equipment; Evaluation; Florida; Fluorescein-5-isothiocyanate; Fluorescence; Fluorescence Microscopy; Follow-Up Studies; Freezing; G Protein-Coupled Receptor Genes; Generic Drugs; Goals; Hand; Hour; Housing; Image; Image Analysis; Incubators; Informatics; Label; Laboratories; Lactamase; Lasers; Length; Life; Maintenance; Measurement; Measures; Mentors; Microinjections; Microscope; Microscopy; Miniaturization; Molecular and Cellular Biology; Mycoplasma; Nucleic Acids; Outsourcing; Peptides; Performance; Procedures; Process; Production; Proteins; Protocols documentation; Provider; PubChem; Quality Control; Readiness; Reagent; Records; Reporter; Reporting; Research Personnel; Resources; Robotics; Running; Sampling; Scanning; Schedule; Science; Screening procedure; Secure; Source; Specificity; Stream; Summary Reports; Surveys; Synthesis Chemistry; System; Teleconferences; Temperature; Testing; Time; TimeLine; Titrations; United States National Institutes of Health; Update; Vendor; Vial device; Visual; Work; Workplace; assay development; base; continuous cell line; counterscreen; enzyme substrate; fluorescence microscope; follow-up; foot; inhibitor/antagonist; interest; meetings; member; micromanipulator; outreach; release of sequestered calcium ion into cytoplasm; response; scale up; square foot; tissue culture

ASSAY DEVELOPMENT/ADAPTATION CORE In La Jolla, 600 square ft of laboratory space is dedicated to the MLPCN effort. The lab is configured for maximal use of space with the tissue culture room adjacent to the automation bench. The tissue culture room has two 6-foot biosafety cabinets. In addition, the PI has 2500 sq. ft lab space immediately below the assay development facility. The lab is fully equipped for cell and molecular biology and is used in assay development efforts requiring these capabilities. The tissue culture lab has a Zeiss Axiovert fluorescent microscope equipped with filters for visual detection of green fluorescence (FITC, GFP) and CCF2/4 (b-lactamase substrate) loaded cells. The ICND building has a core microscopy facility available for secondary assays (e.g. GPCR internalization) with the following equipment. ¿ Brightfield and fluorescence microscopes ¿ Dissecting stereoscope with color camera ¿ Calcium imaging automated system with micromanipulator for cell picking Olympus confocal laser-scanning microscope with 4 laser lines ¿ DeltaVision deconvolution acquisition system ¿ Spinning-disk confocal microscope (2 laser lines) with incubator chamber for live-cell fluorescence microscopy ¿ Live-cell brightfield microscope with incubator ¿ Eppendorf microinjection system on inverted fluorescence microscope ¿ MetaMorph 6.5 acquisition and image analysis software

检测开发/适应核心 在拉荷亚，600 平方英尺的实验室空间专门用于 MLPCN 工作。 组织培养室与自动化工作台相邻，最大限度地利用空间。 有两个6英尺生物安全柜。 此外，PI 在检测开发设施下方拥有 2500 平方英尺的实验室空间。 配备用于细胞和分子生物学，并用于需要这些功能的分析开发工作。 组织培养实验室拥有配备滤光片的 Zeiss Axiovert 荧光显微镜，用于视觉检测 绿色荧光（FITC、GFP）和 CCF2/4（b-内酰胺酶底物）负载细胞。 ICND 大楼拥有可用于二次分析（例如 GPCR 内化）的核心显微镜设施 与以下设备。 ¿明场和荧光显微镜 ¿用彩色相机解剖立体镜 ¿带有用于细胞拾取的显微操作器的钙成像自动化系统 奥林巴斯共焦激光扫描显微镜，带 4 条激光线 ¿ DeltaVision 反卷积采集系统 ¿转盘共焦显微镜（2 条激光线），带活细胞荧光培养箱 显微镜检查 ¿带培养箱的活细胞明场显微镜 ¿倒置荧光显微镜上的 Eppendorf 显微注射系统 ¿ MetaMorph 6.5 采集和图像分析软件