Investigations and Mitigation of Carbon Nanomaterial Toxicity
碳纳米材料毒性的调查和减轻
基本信息
- 批准号:7983281
- 负责人:
- 金额:$ 41.11万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-09-01 至 2015-04-30
- 项目状态:已结题
- 来源:
- 关键词:Active SitesAffectAnimalsApoptosisAreaAsbestosAutomobile DrivingBiodegradationBiologicalBone MarrowBreathingCarbonCarbon NanotubesCatalysisCellsCharacteristicsChemicalsCommunitiesDataDevelopmentEcosystemEnvironmentEnzymesExcisionExhibitsFiberFutureGoalsGrantHandHealthHealth HazardsHigh Pressure Liquid ChromatographyHorseradish PeroxidaseHumanHydrogen PeroxideHydrophobic InteractionsImmune systemImmunoglobulin GIncinerationInflammationInvadedInvestigationKineticsLaboratoriesLungMethodsMolecularNMR SpectroscopyNanotechnologyNanotubesOrganismOxidative StressOxygenParticulatePeroxidasesPhospholipidsPhysiologicalPlantsPlayProcessProductionProtocols documentationReagentReportingResearchRiskRiversRoleSkinSourceStreamSurfaceTemperatureTestingTissuesToxic effectbasecarboxylatecofactorcommercializationfunctional groupin vivoinfrared spectroscopykillingslarge scale productionmicroorganismnanomaterialsneutrophilnew technologynovelprogramspublic health relevanceremediationscale upsurface coatingwasting
项目摘要
DESCRIPTION (provided by applicant): As carbon nanotube (CNT) applications become more abundant, so do production demands. Because of this increased activity, much research has been performed in examining the toxicity of CNTs. Nanotubes have been found to exhibit toxic effects to the body including inflammation, oxidative stress, and apoptosis, similar to that witnessed from asbestos. As such, an effective method to remove these toxic fibers from the environment needs to be developed. We hypothesize here that by using enzymatic catalysis, we can initiate degradation of CNTs in environmentally and physiologically relevant settings. Our preliminary data shows that a natural enzyme horseradish peroxidase (HRP), along with low, local concentrations of H2O2 (40 5M), can degrade these nanomaterials. We have also observed that human myeloperoxidase (hMPO) can degrade CNTs, illustrating a two-pronged approach (environment and human health). Our specific goals for this project include (1) identifying environmental and structural factors affecting the kinetics of CNT degradation using HRP and hMPO, (2) identifying the molecular mechanism(s) of CNT degradation catalyzed by HRP and hMPO, (3) determining the products of HRP/hMPO- degradation of CNTs and evaluating their toxicity. The significant endpoint of this study will be marked by an understanding of the effects of CNTs and their transformation products on human health, including the necessary chemical functionalization for their biodegradation in the environment and in vivo.
PUBLIC HEALTH RELEVANCE: Developments in nanotechnology vastly increase the demand and use of carbon nanotubes, correspondingly with their health hazards and toxicity to the environment. This dictates the necessity to develop approaches for safe removal of carbon nanotubes from the environment. This proposal outlines novel technologies and protocols for biodegradation of carbon nanotubes through enzymatic catalysis mitigating environmental and subsequent physiological toxicity from carbon nanomaterials.
描述(申请人提供):随着碳纳米管(CNT)的应用越来越丰富,生产需求也越来越丰富。由于这种活性的增加,人们进行了大量研究来检查碳纳米管的毒性。人们发现纳米管对身体具有毒性作用,包括炎症、氧化应激和细胞凋亡,与石棉的作用类似。因此,需要开发一种有效的方法来从环境中去除这些有毒纤维。我们假设通过使用酶催化,我们可以在环境和生理相关环境中引发碳纳米管的降解。我们的初步数据表明,天然酶辣根过氧化物酶 (HRP) 以及局部低浓度的 H2O2 (40 × 5M) 可以降解这些纳米材料。我们还观察到人髓过氧化物酶(hMPO)可以降解碳纳米管,这说明了双管齐下的方法(环境和人类健康)。我们该项目的具体目标包括 (1) 确定影响 HRP 和 hMPO 降解 CNT 动力学的环境和结构因素,(2) 确定 HRP 和 hMPO 催化 CNT 降解的分子机制,(3) 确定HRP/hMPO-碳纳米管降解产物并评估其毒性。这项研究的重要终点将是了解碳纳米管及其转化产物对人类健康的影响,包括其在环境和体内生物降解所需的化学功能化。
公共健康相关性:纳米技术的发展极大地增加了碳纳米管的需求和使用,相应地增加了碳纳米管对健康的危害和对环境的毒性。这表明有必要开发从环境中安全去除碳纳米管的方法。该提案概述了通过酶催化生物降解碳纳米管的新技术和方案,减轻碳纳米材料的环境毒性和随后的生理毒性。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(2)
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{{ truncateString('Alexander Star', 18)}}的其他基金
Investigations and Mitigation of Carbon Nanomaterial Toxicity
碳纳米材料毒性的调查和减轻
- 批准号:
8134365 - 财政年份:2010
- 资助金额:
$ 41.11万 - 项目类别:
Investigations and Mitigation of Carbon Nanomaterial Toxicity
碳纳米材料毒性的调查和减轻
- 批准号:
8272658 - 财政年份:2010
- 资助金额:
$ 41.11万 - 项目类别:
Investigations and Mitigation of Carbon Nanomaterial Toxicity
碳纳米材料毒性的调查和减轻
- 批准号:
8463530 - 财政年份:2010
- 资助金额:
$ 41.11万 - 项目类别:
Investigations and Mitigation of Carbon Nanomaterial Toxicity
碳纳米材料毒性的调查和减轻
- 批准号:
8668952 - 财政年份:2010
- 资助金额:
$ 41.11万 - 项目类别:
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