Animal Models Core

动物模型核心

基本信息

批准号：
8134873
负责人：
G IAN GALLICANO
金额：
$ 17.56万
依托单位：
UNIVERSITY OF TX MD ANDERSON CAN CTR
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8134873
关键词：
Animal Model Animals Antineoplastic Agents Archives Biological Assay Breeding Cancer Patient Caring Cataloging Catalogs Cell Separation Cells Clinical Clinical Research Computer software Coupled Cryopreservation Data Databases Embryo Ensure Equipment Excision Fetus Fibroblasts Freezing Genetic Genotype Growth Hand Health Housing Human Image Immunity Individual Injection of therapeutic agent Intestinal Cancer Knock-out Knockout Mice Liquid substance Location Longitudinal Studies Malignant Neoplasms Malignant neoplasm of large intestine Medical Genetics Mission Modeling Mus Nitrogen Nude Mice Online Systems Operating Rooms Operative Surgical Procedures Outcome Pancreas Partner in relationship Patients Pharmaceutical Preparations Pharmacotherapy Predisposition Primitive foregut structure Printing Production Publishing Reaction Records Research Research Personnel Research Project Grants Resolution Role Running Sampling Schedule Services Site Small Intestines Sorting - Cell Movement Source Specific Pathogen Frees Stomach Testing Tissues Transgenic Mice Transgenic Model Transgenic Organisms Translational Research Tumor Tissue Ultrasonography Universities Work Xenograft procedure blastocyst chemotherapeutic agent cost efficacy testing embryonic stem cell germ free condition in vivo malignant stomach neoplasm member mouse model mutant neoplastic cell offspring programs pup sperm cell tissue preparation tumor

项目摘要

The Animal Models Core (AMC) will support all of the research projects in the program by providing the necessary genetically modified mice and producing low-passage in vivo human gastro-intestinal cancer explants in mice. One mission of the AMC will be to maintain mice for long-term studies to evaluate predisposition to cancer. A second mission of the AMC will be maintaining stocks of the genetically modified strains (e.g., elf, eif/Smad4, Smad4, Smad3, Cdk4-R24C mutant, cdk4 null mutant, htert knockout), and to breed these strains together to produce fetuses of double and triple mutant homozygous genotypes. Working with the Tissue Core B, these fetuses will be used as a source of embryonic fibroblasts and tumor cells by the projects. Pups with the desired double or triple mutant genotypes will constitute a large fraction of the progeny. To provide a continuous supply in support of the research projects a large amount of breeding, coupled with genotype analysis to identify the appropriate individuals will be required. A third mission of the AMC will be to provide synergy between the clinicians and projects testing anti-cancer drug therapies. This synergy will be in the form of producing, and providing husbandry of mice harboring genetic backgrounds predisposed to making tumors or nude mice xenografted with human tumor tissues for propagating lowpassage tumors. All mice will be produced and housed under barrier conditions that ensure continuing specific pathogen free status, Centralization of the production and care of these mice under the AMC will limit the number of individuals handling the animals, thereby further safeguarding their health. Moreover, by centralizing the production and dispensation of these genetically valuable animals, the animal core will be able to coordinate usage among the different projects, avoid wastage, and achieve an economy of scale that will permit these studies to be completed at less expense than if undertaken separately. A final mission of the AMC will be to serve as a synergistic hub for all projects and cores.

动物模型核心（AMC）将通过提供计划中的所有研究项目必要的转基因小鼠，并在体内胃肠道癌中产生低通量小鼠的外植体。 AMC的一个任务将是维护老鼠以进行长期研究以评估癌症的易感性。 AMC的第二个任务将维持基因修改的库存菌株（例如ELF，EIF/SMAD4，SMAD4，SMAD3，CDK4-R24C突变体，CDK4 Null突变体，HTERT敲除）和TO 将这些菌株繁殖在一起，产生双重和三重突变体纯合基因型的胎儿。在职的使用组织核B，这些胎儿将用作胚胎成纤维细胞和肿瘤细胞的来源项目。所需的双重或三重突变基因型的幼崽将构成很大一部分后代。为了支持研究项目的持续供应，大量繁殖，结合基因型分析以确定适当的个体。第三个任务 AMC将在临床医生和测试抗癌药物疗法的项目之间提供协同作用。这协同作用将以生产形式，并提供具有遗传背景的老鼠的饲养易于制造用人肿瘤组织进行肿瘤或裸鼠异种移植以传播低通道肿瘤。所有老鼠将在障碍条件下生产和容纳，以确保继续特定的无病原体状态，在AMC下的这些小鼠的产生集中和护理将限制处理动物的个人数量，从而进一步保护其健康。而且，集中这些遗传有价值动物的生产和分配，动物核心将是能够协调不同项目之间的用法，避免浪费并实现规模经济的经济将允许这些研究以比单独进行的费用更少的费用完成。最后一个任务 AMC将充当所有项目和核心的协同枢纽。