ATHYMIC ANIMAL AND XENOG

无胸动物和异种

• 批准号: 7529375
• 负责人: LILI LIU
• 金额: $ 10.09万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-06 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7529375
• 关键词: Animal Housing; Animal Model; Animals; Antineoplastic Agents; Biological Models; Breeding; Cancer Center; Cells; Class; Comprehensive Cancer Center; Consultations; Containment; Core Facility; Cytotoxic T-Lymphocytes; Data; Drug Kinetics; Educational process of instructing; Environment; Equipment; Evaluation; Gene Expression; Genes; Health; Housing; Human; Image; Immune; Immunodeficient Mouse; Implant; Injection of therapeutic agent; Location; Malignant Neoplasms; Measurement; Mission; Monitor; Mus; Neoplasm Metastasis; Nude Mice; Operative Surgical Procedures; Pharmaceutical Preparations; Pharmacologic Substance; Principal Investigator; Procedures; Quarantine; Rattus; Reporting; Research Personnel; SCID Mice; Services; Site; Testing; Training; Transportation; Tumor Antigens; Vendor; Xenograft procedure; angiogenesis; cytokine; drug distribution; in vivo; model development; mouse model; neoplastic cell; pathogen; preclinical study; research study; response; size; tumor; tumor growth; tumor xenograft

The Athymic Animal & Xenograft Core Facility includes two sites, one at Case and one at the Taussig Cancer Center. The core is directed by Dr. Lili Liu and co-directed by Dr. Daniel Lindner, who oversees the Taussig facility. The core provides a pathogen-free environment for breeding and housing athymic immune deficient mice and rats for preclinical studies. A breeding colony for nude mice (NCR nu/nu) has been established to provide animals to investigators without quarantine. Investigators can also purchase other immune-deficient classes of animals, such as SCID mice, Rag 2 mice and nude rats, from external vendors and have them housed in the core for subsequent experiments after a quarantine period. Investigators use mice in the facility for studies of human tumor xenografts to test anticancer drug effects on syngeneic murine tumor implants, to determine species-specific effect of cytokines and evaluate cytotoxic T cell (CTL) response to tumor-associated antigens; to test oncogenicity of specific tumor genes on human tumor xenografts; and to perform small animal imaging to detect cancer size and location, as well as anticancer drug distribution and pharmacokinetic study in vivo. The facility provides support services for maintaining xenografts of human cancers in mouse model systems, as well as training and assistance to Cancer Center investigators; equipment and services for performing various orthotopic or ectopic injections; surgical expertise for reversible procedures; and a high-containment and safe environment for investigators using Pharmaceuticals and anticancer agents in these animal model systems. The facility helps in evaluation of new pharmacologic agents by providing data on tumor growth, metastasis, angiogenesis, and gene expression. The facility also provides consultation services to assist the investigator in all aspects of animal tumor model development.

无胸腺动物和异种移植核心设施包括两个地点，一处位于 Case，一处位于 Taussig 癌症中心。核心由 Lili Liu 博士指导，Daniel Lindner 博士联合指导，Daniel Lindner 博士负责监督 陶西格设施。核心为无胸腺免疫动物的繁殖和饲养提供了无病原体的环境 缺陷小鼠和大鼠用于临床前研究。裸鼠 (NCR nu/nu) 的繁殖群体已建成 设立的目的是向调查人员提供未经检疫的动物。调查人员还可以购买其他 来自外部供应商的免疫缺陷动物类别，例如 SCID 小鼠、Rag 2 小鼠和裸鼠 并在隔离期后将它们安置在核心中进行后续实验。调查人员使用 人体肿瘤异种移植研究设施中的小鼠，以测试抗癌药物对同基因小鼠的作用 肿瘤植入物，以确定细胞因子的物种特异性效应并评估细胞毒性 T 细胞 (CTL) 对肿瘤相关抗原的反应；测试特定肿瘤基因对人类肿瘤的致癌性 异种移植物；并进行小动物成像以检测癌症大小和位置，以及抗癌 体内药物分布和药代动力学研究。 该设施为在小鼠模型系统中维持人类癌症异种移植物提供支持服务， 以及对癌症中心研究人员的培训和协助；表演设备和服务 各种原位或异位注射；可逆手术的外科专业知识；以及高遏制 为研究人员在这些动物模型中使用药物和抗癌药物提供安全的环境 系统。该设施通过提供肿瘤生长的数据来帮助评估新的药物制剂， 转移、血管生成和基因表达。该设施还提供咨询服务，以协助 动物肿瘤模型开发各个方面的研究者。