Cell Imaging Core

细胞成像核心

• 批准号: 8287601
• 负责人: ROBERT B VERNON
• 金额: $ 25.92万
• 依托单位: BENAROYA RESEARCH INST AT VIRGINIA MASON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8287601
• 关键词: Adoptive Transfer; Antibodies; Bacterial Infections; Cell Separation; Cells; Consultations; Dyes; Equipment; Extracellular Matrix; Fibrosis; Flow Cytometry; Histology; Human Resources; Image; Immune; Inflammation; Leukocytes; Lung; Lymphocyte; Methods; Microscope; Microscopy; Nucleic Acid Probes; Pathology; Photomicrography; Pneumonia; Population; Preparation; Process; Protocols documentation; Regulation; Research Personnel; Sampling; Services; Slide; Sorting - Cell Movement; Staining method; Stains; Stromelysin 1; TSLP gene; Training; Virus Diseases; Work; cellular imaging; digital; experience; instrument; macrophage; research study; response; tissue fixing; tissue processing; trafficking

Core C will utilize the equipment and staff of the existing BRI Histology and Flow Cytometry Cores to provide histological processing, photomicrography, and flow cytometry services for the four Projects described in this proposal. For histology. Core C will provide high-throughput equipment for tissue processing, embedding, sectioning, and staining with conventional dyes, antibodies, and nucleic acid probes. For photomicrography, Core C will provide one confocal microscope and five digital camera-equipped conventional microscopes with complementary imaging capabilities. For flow cytometry, Core C will incorporate a variety of multifeatured cell sorting instruments. With regard to human resources, Core C will have experienced staff who will provide Project personnel with a full range of histological prep, consultation, and instrument training services

核心C将利用现有的BRI组织学和流式细胞仪的设备和人员提供 为此描述的四个项目的组织学处理，显微照片和流式细胞仪服务 提议。用于组织学。 Core C将提供用于组织加工，嵌入的高通量设备 切片，并用常规染料，抗体和核酸探针染色。对于显微照片， Core C将提供一个共聚焦显微镜和五个配备数码相机的常规显微镜 具有互补的成像功能。对于流式细胞仪，Core C将结合各种多功能 细胞分类仪器。关于人力资源，核心C将拥有经验丰富的员工 将为项目人员提供各种组织学准备，咨询和仪器培训 服务