Cell Culture Core

细胞培养核心

• 批准号: 8318833
• 负责人: CHINNASWAMY TIRUPPATHI
• 金额: $ 24.71万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8318833
• 关键词: 4-ethoxymethylene-2-phenyl-2-oxazoline-5-one; Acute Lung Injury; Address; B-Lymphocytes; Blood; Bone Marrow; Cell Culture Techniques; Cell Separation; Cells; Communication; Core Facility; Disease; Dreams; Endothelial Cells; Goals; Human; Human Resources; Inflammatory; Injury; Knock-out; Knockout Mice; Lung; Lung Inflammation; Mediating; Methods; Mus; NADPH Oxidase; Protein Kinase C Alpha; Quality Control; Reagent; Services; Signal Transduction; Source; Training; Transgenic Organisms; Vascular Endothelial Cell; cost effectiveness; design; genetic manipulation; knock out mouse project; lung injury; lung vascular injury; meetings; neutrophil; new therapeutic target; programs; pulmonary artery endothelial cell; research study; success; sulfated glycoprotein 2

Core B is the central cell culture facility, which will provide support for each of four projects. This core facility will provide services for the following: 1) isolation of neutrophils (PMNs) from mouse blood and bone marrow; 2) isolation, characterization, and culture of mouse lung endothelial cells, and 3) culture of human lung microvascular and pulmonary artery endothelial cells for the experiments involved in the Projects as outlined in the Core description below. Mouse lung endothelial cell isolation and culture is pivotal for the accomplishment of studies proposed in Projects 1, 2, 3, and 4. In addition. Core B will also isolate PMNs from mouse blood and bone marrow for Projeds 1, 2, and 3. A major task of this core will be to provide lung endothelial cells and PMNs from different knock-out mice; e.g., TRPM2 knock-out, NADPH oxidase subunit gp91(phox) knock-out, and PKC-alpha knock-out mice for Project 1; MKP5 knock-out, p47(phox) knock-out, MK2 knock-out, and MKP5/MK2-double knock-out mice for Project 2; TRPC4 knock-out; TRPM2 knock-out, and Dream knock-out mice for Project 3; FoxMI transgenic and iCKO mice for Project 4. Thus the adivity of this Core is essential for the success of the Program Projed studies.

核心 B 是中央细胞培养设施，将为四个项目中的每一个项目提供支持。该核心设施将提供以下服务：1）从小鼠血液和骨髓中分离中性粒细胞（PMN）； 2) 小鼠肺内皮细胞的分离、表征和培养，以及 3) 人肺微血管和肺动脉内皮细胞的培养，用于项目所涉及的实验，如下面的核心描述中所述。小鼠肺内皮细胞分离和培养对于完成项目 1、2、3 和 4 中提出的研究至关重要。此外。 Core B 还将从小鼠血液和骨髓中分离 PMN，用于 Projeds 1、2 和 3。该核心的主要任务是提供来自不同基因敲除小鼠的肺内皮细胞和 PMN；例如，项目1的TRPM2敲除、NADPH氧化酶亚基gp91(phox)敲除和PKC-α敲除小鼠；项目2的MKP5敲除、p47(phox)敲除、MK2敲除和MKP5/MK2双敲除小鼠； TRPC4 敲除；项目 3 的 TRPM2 敲除小鼠和 Dream 敲除小鼠；用于项目 4 的 FoxMI 转基因小鼠和 iCKO 小鼠。因此，该核心的活性对于计划项目研究的成功至关重要。