CELL SURFACE GLYCOSPHINGOLIPIDS SSEA-3 AND SSEA-4

细胞表面糖脂 SSEA-3 和 SSEA-4

• 批准号: 7722618
• 负责人: ALFRED Harrison MERRILL
• 金额: $ 0.94万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-08 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722618
• 关键词: Carbohydrates; Cell surface; Cells; Computer Retrieval of Information on Scientific Projects Database; Development; Epitopes; Funding; Grant; Human; Human Development; In Vitro; Institution; Modeling; Molecular; Population; Proteoglycan; Research; Research Personnel; Resources; Source; Sphingolipids; Staging; Testing; Undifferentiated; United States National Institutes of Health; blastocyst; embryonic antigen; embryonic stem cell; extracellular; stage-specific embryonic antigen 4

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Pluripotent cells can be isolated from the human blastocyst and maintained in culture as self-renewing, undifferentiated, embryonic stem cells (hESCs). They are a valuable model of human development in vitro, and are the focus of substantial research aimed at generating differentiated populations for cellular therapies. The extracellular markers that have been used to characterize hESCs are primarily carbohydrate epitopes on proteoglycans or sphingolipids, such as the stage-specific embryonic antigens (SSEA)-3 and 4. The expression of SSEA-3 and 4 is tightly regulated during preimplantation development and on hESCs. While this might imply a molecular function in undifferentiated cells, it has not yet been tested experimentally.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 多能细胞可以从人类囊胚中分离出来，并在培养物中维持自我更新， 未分化的胚胎干细胞（hESC）。它们是人类体外发育的有价值的模型， 大量研究的重点旨在为细胞疗法产生差异化人群。这 用于表征 hESC 的细胞外标记主要是碳水化合物表位 蛋白聚糖或鞘脂，例如阶段特异性胚胎抗原 (SSEA)-3 和 4。的表达式为 SSEA-3 和 4 在植入前发育过程中和 hESC 上受到严格调控。虽然这可能意味着 未分化细胞中的分子功能，尚未经过实验测试。