STUDIES OF YEAST CDC14

酵母的研究 CDC14

• 批准号: 7722218
• 负责人: FREDERICK R. CROSS
• 金额: $ 0.33万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722218
• 关键词: Antibodies; Cell Cycle; Cell Cycle Progression; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Housing; Institution; Localized; Methods; Phosphoric Monoester Hydrolases; Proteins; Research; Research Personnel; Resources; Source; United States National Institutes of Health; Yeasts; magnetic beads; mutant

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We use our newly developed method for the isolation of GFP-tagged proteins to study the cell-cycle dependent interactions of the phosphatase, Cdc14,required for cell cycle progression. Large scale yeast cultures were grown for two mutants of Cdc14, one localized at the spindle pole and the other with cytoplasmic localization. We have performed isolations using magnetic beads coated with our in-house developed anti-GFP antibodies and identified Cdc14-interacting partners specific for each strain.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们使用新开发的分离 GFP 标记蛋白的方法来研究细胞周期进展所需的磷酸酶 Cdc14 的细胞周期依赖性相互作用。对两种 Cdc14 突变体进行大规模酵母培养，一种位于纺锤体极，另一种位于细胞质。 我们使用涂有我们内部开发的抗 GFP 抗体的磁珠进行分离，并确定了每种菌株特异的 Cdc14 相互作用伙伴。