ANIMAL CORE

动物核心

• 批准号: 8381688
• 负责人: JAMES C. ROSE
• 金额: $ 34.84万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-20 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8381688
• 关键词: Acetates; Acute; Adolescent; Adult; Age; Anesthesia procedures; Animal Model; Animals; Autopsy; Betamethasone; Body Weight; Breeding; Chronic; Data; Databases; Dose; Endocrinology; Ensure; Event; Gays; Gender; Goals; Growth; Hour; Human; In Vitro; Individual; Injection of therapeutic agent; Instruction; Knowledge; Laboratory Research; Lung; Modeling; Operative Surgical Procedures; Perinatal; Physiologic Monitoring; Physiological; Physiology; Plasma; Play; Pregnancy; Randomized; Research Personnel; Research Project Grants; Role; Sampling; Sheep; Stress; Time; Tissue Banks; Tissues; Translating; Work; age group; animal resource; base; experience; fetal; in vivo; inorganic phosphate; instrument; offspring; operation; postnatal; pregnant; prenatal; research study

The Animal Core provides the investigators with chronically instrumented sheep and with non instrumented pregnant and nonpregnant animals. The specific goals of the Animal Core are: to obtain time- dated pregnant animals at 75 days of gestation; to prepare chronically instrumented juvenile and adult sheep for use in the different research projects; to conduct necropsies and facilitate the collection of tissues; and to facilitate the in vivo studies involving monitoring physiologic variables under acute or chronic conditions. Pregnant ewes are randomly assigned to receive either vehicle or betamethasone injections at 80 an 81 days gestation and lambs are allowed to deliver spontaneously at term. The Animal Core is essential for three of the research projects. The director of the core has more than 25 years of experience working with chronically instrumented fetal and pregnant animals. The Core provides the unique combination of the ability to infuse physiological amounts of putative regulators, the availability of plasma samples, in vivo recording of physiological variables and tissues for in vitro studies from the same animal in which in vivo studies have been conducted. Sheep will be chronically instrumented in order to obtain physiological data in animals that are not under the stress of anesthesia. We will use fime dated pregnant sheep provided to us by Hash, Inc. The supplies maintains a very large flock and has consistently provided us with healthy and accurately dated pregnant animals. All of these surgeries and manipulafions will be conducted within the Animal Core in the Perinatal Research Laboratory. Pregnant and adult sheep of both genders will be used. Betamethasone is administered in a manner similar to that used for stimulafing lung maturafion in the unborn human, i.e., two doses of a mixture of the acetate and phosphate form, given 24 hours apart. The only difference is that we will adjust for maternal body weight and give 0.17 mg/kg with a maximum of 12 mg/day.

动物核心为研究人员提供了长期仪器的绵羊，并提供了非仪器的怀孕和未怀孕的动物。动物核心的具体目标是：在妊娠75天内获得时代过时的怀孕动物；为长期用仪器的少年和成年绵羊准备在不同的研究项目中使用；进行尸检并促进组织的收集； 并促进涉及监测急性或慢性条件下生理变量的体内研究。怀孕的母羊被随机分配为80 A A A 81天妊娠的车辆或倍他米松注射，并允许羔羊在期间自发交付。 动物核心对于三个研究项目至关重要。核心主任拥有25年以上的经验，可用于长期仪器的胎儿和孕妇。核心提供了注入生理量的推定调节剂，血浆样品的可用性，生理变量和组织的体内记录的独特组合 从已经进行了体内研究的同一动物中。 绵羊将经过长期仪器，以获取不受麻醉压力的动物的生理数据。我们将使用Hash，Inc。提供给我们的冰期过时的怀孕绵羊。这些物资保留了一只非常大的羊群，并一直为我们提供健康且准确的日期怀孕 动物。所有这些手术和操作将在围产期研究实验室的动物核心内进行。将使用两种性别的孕妇和成年绵羊。倍替米松的给药类似于刺激未出生的人的肺部成熟的方式，即乙酸盐和磷酸盐形式的两种混合物，相距24小时。唯一的区别是，我们将针对母体体重进行调整，并给出0.17 mg/kg，最多为12 mg/天。