Translational Methods/Facilities Core (TMF - Core) (8 of 8)

转化方法/设施核心（TMF - 核心）（8 / 8）

• 批准号: 8307668
• 负责人: CHRISTOPHER J. EVANS
• 金额: $ 7.5万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-30 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8307668
• 关键词: Abnormal coordination; Animal Model; Behavior assessment; Behavioral Assay; Biocompatible Materials; Biological; Biological Assay; California; Candidate Disease Gene; Cells; Collaborations; Communities; Complex; Consultations; Core Facility; DNA; Data; Databases; Development; Ensure; Etiology; Faculty; Genes; Genetic; Genetic Variation; Genome; Genomics; Genotype; Goals; Human; International; Investigation; Mainstreaming; Methods; Modeling; Mus; Phenotype; Population; Preparation; Process; Proteins; RNA; Research; Research Activity; Research Design; Research Personnel; Resources; Rodent; Sampling; Testing; Time; Transgenes; Transgenic Organisms; Universities; base; cost; data management; design; genetic analysis; genetic association; genome-wide; member; memory process; model development; mouse model; mutant; neuropsychiatry; novel; operation; phenomics; programs; response; success; vervet

The Translational Methods/Facilities (TMF) Core will provide state of the art genetic analysis as well as mouse model development, characterization and assessment for all components of the Consortium for Neuropsychiatric Phenomics. Three distinct but interactive units operationally define the specific aims of the TMF-Core: ¿ The Genetic Studies Unit will be responsible for generating and analyzing all genetic data in the multiple association studies to be conducted by the Consortium. ¿ The BAG Transgenic Unit will create novel murine models using BAG transgenes or by crossing established mutant lines to a) test the cellular etiology of genotype/phenotype associations by genetic rescue strategies, b) drive cell-specific expression of fluorescent proteins to identify CNS circuitry that may be perturbed via mutant genes and c) model the contribution of candidate genes identified and/or anticipated from ongoing genetic association analysis to significantly modify memory processes or response inhibition. ¿ The Rodent Phenotyping Unit in close collaboration with the Human Translational Applications Core will design, validate and conduct behavioral assays to phenotype mouse models used by the Consortium. The three units leverage considerable physical and intellectual resources at UCLA. Such leveraging will enable the TMF-Core to cost-effectively and efficiently provide informational genetic/phenotype databases, shared animal models and validated behavioral assessments throughout the Consortium and subsequently to the research community outside of UCLA.

翻译方法/设施（TMF）核心将提供最先进的遗传分析以及 对财团的所有组成部分的小鼠模型开发，表征和评估 神经精神现象学。三个不同但交互式单元在操作上定义了特定目的 TMF核： �遗传研究单位将负责生成和分析所有遗传数据 多个关联研究将由财团进行。 »袋子转基因单元将使用袋子转基因或越过创建新颖的鼠模型 建立的突变型线以a）测试遗传学的基因型/表型关联的细胞病因 救援策略，b）驱动荧光蛋白的细胞特异性表达，以识别CNS电路 可以通过突变基因和c）模拟鉴定的候选基因的贡献 和/或从持续的遗传关联分析中预期的，以显着修改记忆 过程或响应抑制。 �啮齿动物表型单元与人类翻译应用密切合作 将设计，验证和进行行为测定到由鼠标模型的表型小鼠模型 财团。 这三个单元在UCLA利用了可观的物理和智力资源。这样的杠杆意愿 启用TMF核心能够具有成本效益有效地提供信息遗传/表型数据库，即 共享动物模型和整个财团的验证行为评估，随后 到加州大学洛杉矶分校以外的研究社区。