Effect of Vocalization on Tissue Protein Levels in the Acute Vocal Fold Wound

发声对急性声带伤口组织蛋白水平的影响

基本信息

批准号：
7390236
负责人：
Bernard Rousseau
金额：
$ 7.68万
依托单位：
VANDERBILT UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-04-01 至 2010-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7390236
关键词：
Acute Agreement Animal Experimentation Animal Experiments Animal Model Brain Caring Cicatrix Clinical Collagen Collection Complication Data Deposition Development Dose Electric Stimulation Electrodes Epithelial Extracellular Matrix Fibronectins Gene Expression Genes Glycosaminoglycans Goals Hour Hyaluronan Image Analysis Impaired wound healing In Vitro Injury Investigator-Initiated Research Lamina Propria Measures Mechanics Methods Midbrain structure Modeling Nerve Operative Surgical Procedures Oryctolagus cuniculus Patient Care Phonation Postoperative Period Preparation Proteins Recurrence Relative (related person)Reporting Research Rest Series Staining method Stains Standards of Weights and Measures Structure of superior laryngeal nerve Techniques Time Tissue Sample Tissues Trauma Voice Wound Healing Writing base in vivo injured midbrain central gray substance programs research study theories tissue processing vibration vocal cord vocalization wound

项目摘要

DESCRIPTION (provided by applicant): The effect of vocal fold vibration on remodeling of the vocal fold after injury is poorly understood. Clinically, this has resulted in very little agreement with regard to post-operative voice use, voice rest, and lack of a standard of clinical care for patients undergoing phonomicrosurgery. Historically, support for voice rest has been driven by theories that implicate phonation associated trauma with delayed wound healing. In contrast, recent in-vitro studies have shown that gene expression levels of extracellular matrix genes respond favorably to mechanical load. In this application we will 1) Elicit vocalization in a rabbit animal model post-wounding and 2) measure the effects of a 35 minute and 120 minute dose of vocalization on gene expression and tissue levels of the protein products pro-collagen I, collagen, fibronectin, and the glycosaminoglycan hyaluronan prior to epithelial coverage of the wounded vocal fold. We will demonstrate that brain and nerve stimulation techniques provide suitable methods for eliciting vocalization in the rabbit. We hypothesize that 120 minutes of vocalization initiated 72 hours after injury results in significantly elevated levels of fibronectin and collagen, while 35 minutes of vocalization initiated 72 hours after injury results in stable levels of pro-collagen I, collagen, fibronectin, and hyaluronan relative to control. These experiments will result in the development of a rabbit in- vivo phonation model and rationale for a dosing strategy to be used in the next series of studies that will investigate the effects of vocalization initiated after complete epithelial coverage of the wounded vocal fold on gene expression and extracellular matrix deposition. The long-term objective of this line of research is to understand how vocalization influences organization and remodeling of the injured vocal fold. This research program may have an impact clinically by providing data to support a standard of clinical care regarding post- operative voice use. Project Narrative: Scarring is an adverse complication of vocal fold surgery. The effect of voice use on wound healing of the scarred vocal fold is largely unknown. The goal of this research is to understand how voice use influences wound healing in order to provide an evidence-based standard of care regarding post-operative voice use.

描述（由申请人提供）：声带振动对受伤后声带重塑的影响知之甚少。在临床上，这导致对于术后发声使用、发声休息以及接受声显微手术的患者缺乏临床护理标准方面达成的共识很少。从历史上看，对发声休息的支持是由一些理论推动的，这些理论表明发声相关的创伤会导致伤口愈合延迟。相比之下，最近的体外研究表明，细胞外基质基因的基因表达水平对机械负荷有有利的反应。在此应用中，我们将 1) 在受伤后的兔子动物模型中引发发声，2) 测量 35 分钟和 120 分钟发声剂量对 I 型胶原蛋白前体、胶原蛋白、在上皮覆盖受伤声带之前，加入纤连蛋白和糖胺聚糖透明质酸。我们将证明大脑和神经刺激技术提供了引发兔子发声的合适方法。我们假设受伤后 72 小时开始发声 120 分钟会导致纤连蛋白和胶原蛋白水平显着升高，而受伤后 72 小时开始发声 35 分钟会导致 I 型胶原蛋白前体、胶原蛋白、纤连蛋白和透明质酸水平稳定。控制。这些实验将导致兔子体内发声模型的开发以及在接下来的一系列研究中使用的剂量策略的基本原理，这些研究将调查受伤声带上皮完全覆盖后开始发声对基因表达的影响和细胞外基质沉积。该研究的长期目标是了解发声如何影响受损声带的组织和重塑。该研究项目可能会通过提供数据来支持有关术后语音使用的临床护理标准，从而对临床产生影响。项目叙述：疤痕是声带手术的不良并发症。发声对声带疤痕愈合的影响目前尚不清楚。这项研究的目的是了解声音使用如何影响伤口愈合，以便提供有关术后声音使用的循证护理标准。