Polysaccharide Ecocomposite Materials: Recyclable Synthesis and Applications

多糖生态复合材料：可回收合成及应用

基本信息

批准号：
8230952
负责人：
Chieu D. Tran
金额：
$ 33.91万
依托单位：
MARQUETTE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-05-01 至 2016-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8230952
关键词：
Acids Address Adopted Adsorption Advanced Development Anti-Bacterial Agents Antibiotics Bandage Biocompatible Biocompatible Materials Biological Factors Biomass Biopolymers Blood Cellulose Chemicals Chemistry Chitosan Chloride Ion Chlorides Chlorophenols Ciprofloxacin Cyclodextrins Dependence Development Drug Delivery Systems Ecology Encapsulated Environment Excision Film Fluorescence Fossil Fuels Goals Healed Hemorrhage Hemostatic Agents Hemostatic function High temperature of physical object Hydrogen Hydrogen Bonding In Vitro Iodine Keratin Kinetics Liquid substance Measures Mechanics Methods Monitor One-Step dentin bonding system Organic solvent product Performance Pharmaceutical Preparations Phase Pollution Polymers Polysaccharides Process Property Reaction Reporting Research Science Side Signal Transduction Silver Solutions Solvents Source Structure System Techniques Toxic effect Water Wound Healing absorption antimicrobial base bisphenol A healing improved innovation man next generation novel pollutant research study

项目摘要

DESCRIPTION (provided by applicant): The goal of this project is to systematically and revolutionarily address drawbacks which limit synthesis and applications of composite materials based on the two most abundant biorenewable polysaccharides, cellulose (CEL) and chitotan (CS). The limitations are: (1) the rather costly and multistep process currently used to dissolve CEL and CS which involves strong acid, base and other environmentally harmful chemicals, and (2) the use of man-made polymers to strengthen mechanical properties of CS-based materials; these are not desirable as they may inadvertently alter CS properties, making it less biocompatible, potentially toxic and lessening the unique properties. The goal will be accomplished by (1) exploiting the advantages of ionic liquid (IL), a green solvent, to develop a novel, simple, pollution-free and totally recyclable method to dissolve polysaccharides (CEL, CS and cyclodextrins (CDs)) and keratin without using harmful, volatile organic solvents and/or strong acid and base; (2) using only naturally occurring biopolymers, including polysaccharides and keratin, as support materials to strengthen the structure and expand the utilities while keeping the biodegradable, biocompatible and anti-bacterial properties of CS-based materials intact; and (3) demonstrating that these composite materials are superior to CS-based materials currently available for use as sorbents for pollutants, vehicles to deliver drugs and bandages to control, clean and heal hemorrhages . Butyl methylimmidazolium chloride (BMIm), an IL, will be used to dissolve CEL, CS, CDs and keratin. The resulting IL solutions will then be cast into thin films, and [BMIm] will be removed from the films by soaking the films in water. The final films obtained will contain only all natural biopolymers. Spectroscopic techniques will be used to confirm each step of the synthetic process, to characterize and to determine homogeneity, rheological and mechanical properties of the composite materials prepared. Subsequently, experiments will be carried out to compare these novel composite materials with CS-based materials currently available, to demonstrate that the composite materials have superior properties and hence will advance methods for (1) removing pollutants; (2) maximizing hemostatic properties and (3) delivering drugs. Specifically, the ability of the composite materials to remove pollutants (e.g., bisphenol A and chlorophenols) will be evaluated by measuring kinetics and adsorption isotherms of the adsorption processes by using either fluorescence or UV-visible absorption techniques. Blood absorption and in vitro antimicrobial activity of synthesized CS-based composite materials will be compared to three commercially available bandages, i.e., HemCon bandage (a bandage based on CS), Iodoflex pad (a bandage with iodine) and Silvasorb sheet (bandage with silver). Methods will also be developed to encapsulate ciprofloxacin, an antibiotic drug, into composite materials during the dissolution process with IL and, subsequently, its release from the materials to water will be measured by monitoring the fluorescence signal of ciprofloxacin in the water phase. PUBLIC HEALTH RELEVANCE: The goal of this project is to systematically and revolutionarily address the drawbacks which currently limit synthesis and applications of polysaccharide composite materials, such as cellulose and chitosan. The novel ecocomposite materials obtained through this research will advance the development of innovative and/or improved methods for (1) removal of pollutants; (2) controlling, cleaning and healing hemorrhages and (3) delivering drugs. Moreover, by fabricating new biomaterials from all natural products, the novel polysaccharide ecocomposite materials will be nontoxic, biocompatible, biodegradable and can be used for external as well as internal applications without prior FDA approval, thus leading to a better environment and save lives.

描述（由申请人提供）：该项目的目标是系统地、革命性地解决限制基于两种最丰富的生物可再生多糖——纤维素（CEL）和壳聚糖（CS）——的复合材料的合成和应用的缺点。其局限性在于：(1) 目前用于溶解 CEL 和 CS 的过程相当昂贵且多步骤，其中涉及强酸、强碱和其他对环境有害的化学物质，以及 (2) 使用人造聚合物来增强 CS 的机械性能基础材料；这些都是不可取的，因为它们可能会无意中改变 CS 特性，使其生物相容性较差，具有潜在毒性并削弱其独特特性。该目标将通过（1）利用离子液体（IL）这一绿色溶剂的优势，开发一种新颖、简单、无污染且完全可回收的溶解多糖（CEL、CS和环糊精（CD））的方法。和角蛋白，不使用有害的挥发性有机溶剂和/或强酸和强碱；（2）仅使用天然存在的生物聚合物，包括多糖和角蛋白作为支撑材料，以增强结构并扩大用途，同时保持CS基材料的生物可降解性、生物相容性和抗菌特性； (3)证明这些复合材料优于目前可用作污染物吸附剂、输送药物的载体和控制、清洁和治愈出血的绷带的基于CS的材料。丁基甲基咪唑氯化物 (BMIm) 是一种 IL，将用于溶解 CEL、CS、CD 和角蛋白。然后将所得的离子液体溶液浇铸成薄膜，并通过将薄膜浸泡在水中将[BMIm]从薄膜中除去。最终获得的薄膜将仅包含所有天然生物聚合物。光谱技术将用于确认合成过程的每个步骤，表征和确定所制备的复合材料的均匀性、流变性和机械性能。随后，将进行实验将这些新型复合材料与现有的CS基材料进行比较，以证明该复合材料具有优越的性能，从而将推进（1）去除污染物的方法； (2) 最大化止血特性和 (3) 输送药物。具体而言，将通过使用荧光或紫外-可见吸收技术测量吸附过程的动力学和吸附等温线来评估复合材料去除污染物（例如双酚A和氯酚）的能力。合成的 CS 基复合材料的血液吸收和体外抗菌活性将与三种市售绷带进行比较，即 HemCon 绷带（基于 CS 的绷带）、Iodoflex 垫（含碘绷带）和 Silvasorb 片（含银绷带））。还将开发在IL溶解过程中将抗生素药物环丙沙星封装到复合材料中的方法，随后通过监测水相中环丙沙星的荧光信号来测量其从材料到水中的释放。公共健康相关性：该项目的目标是系统地、革命性地解决目前限制多糖复合材料（如纤维素和壳聚糖）合成和应用的缺点。通过这项研究获得的新型生态复合材料将推动创新和/或改进方法的开发，用于（1）去除污染物； (2) 控制、清理和治愈出血；(3) 输送药物。此外，通过从所有天然产物中制造新型生物材料，新型多糖生态复合材料将具有无毒、生物相容性、可生物降解性，并且无需事先获得 FDA 批准即可用于外部和内部应用，从而创造更好的环境并拯救生命。