Center for the Analysis of Cellular Mechanisms and Systems Biology

细胞机制和系统生物学分析中心

• 批准号: 8230753
• 负责人: EDWARD A DRATZ
• 金额: $ 204.07万
• 依托单位: MONTANA STATE UNIVERSITY - BOZEMAN
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-26 至 2014-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8230753
• 关键词: Advisory Committees; Area; Biological; Biomedical Research; Breathing; Cells; Centers of Research Excellence; Chemicals; Committee Members; Computer software; DNA Microarray Chip; Data; Detection; Development; Digestion; Disease; Educational workshop; Equipment; Faculty; Fourier Transform; Funding; Gel; Genomics; Goals; Grant; Health; Human; Human Resources; Image; Individual; International; Label; Learning; Mass Spectrum Analysis; Measurement; Mentors; Metabolic; Methods; Microfluidics; Modeling; Modification; Molecular; Montana; Neurosciences; Nucleic Acids; Organic Synthesis; Oxidation-Reduction; Pathway interactions; Post-Translational Protein Processing; Productivity; Proteins; Proteomics; Reagent; Recovery; Regulation; Research; Research Infrastructure; Research Personnel; Resolution; Role; Seeds; Services; Signal Transduction; Stimulus; System; Systems Biology; Training; Universities; Work; enzyme activity; improved; liquid chromatography mass spectrometry; member; metabolomics; multidisciplinary; multiplex detection; nano; network models; pathogen; pointed protein; programs; protein complex; research and development; response; tool

The goal of this application is to develop a multidisciplinary Center for the Analysis of Cellular Mechanisms and Systems Biology, to provide new infrastructure to advance understanding of cellular mechanisms, to better understand how to overcome disease, and to build a critical mass of faculty in this area. The Center will be unique in Montana and the five surrounding states, and will include a combination of capabilities for analysis of cellular mechanisms. This project will create a powerful Mass Spectrometry and Proteomics Core infrastructure, oriented toward training researchers. Most cellular mechanisms appear to be controlled by protein post-translational modifications and the MS and Proteomics Core will add: a high resolution Qtof MS for improved "bottom up" characterization of protein post-translational modifications, an FTMS for "top-down" characterization of modifications on intact proteins, a Hyperspectral imager for enhanced multicolor proteomic multiplexing to pin-point proteins that change with biological stimulation, and a protein electro-elution system for high efficiency recovery of pin-pointed proteins. Sensitive differential protein detection capabilities developed at MSU and high sensitivity microfluidic nano LC/MS/MS will be included in the Core. New software will integrate data from an existing DNA microarray Facility. A Proteomics Reagent Synthesis Core will prepare new fluorescent reagents for differential analysis of changes in enzyme activity and post-translational modifications, that result from biological stimulation. We will build critical mass by first supporting the development of three young faculty and one senior faculty member, who is charting a new course in his research. Outstanding local and national mentors and External Advisory Committee members will guide the development of these faculty and the Center. Second, we will hire four new faculty in areas needed to build critical mass: a Chemical Biologist/Organic Synthesis expert, a Biological Mass Spectroscopist using advanced FTMS tools, a Systems Biologist working on metabolic modeling and metabolomics, and a Cell Biologist using global molecular tools. Outstanding MSU Institutional Support ($1,400,000), the significance and timeliness of the post-genomic analysis of cellular mechanisms, and the orientation toward training in advanced methods will seed new projects-and will result in significant scientific contributions and a sustainable Center.

该应用的目的是开发一个多学科的中心，用于分析细胞机制和系统生物学，提供新的基础设施，以提高人们对细胞机制的了解，更好地了解如何克服疾病，并在该领域建立一大批教师。该中心将在蒙大拿州和周围五个状态中是独一无二的，并将包括分析细胞机制的能力。该项目将创建强大的质谱和蛋白质组学核心基础架构，以培训研究人员为导向。 Most cellular mechanisms appear to be controlled by protein post-translational modifications and the MS and Proteomics Core will add: a high resolution Qtof MS for improved "bottom up" characterization of protein post-translational modifications, an FTMS for "top-down" characterization of modifications on intact proteins, a Hyperspectral imager for enhanced multicolor proteomic multiplexing to pin-point proteins that change带有生物刺激和蛋白质电流系统，可高效恢复PIN点蛋白。在MSU上开发的敏感差异蛋白检测能力，高灵敏度微流体Nano LC/MS/MS将包括在核心中。新软件将集成现有的DNA微阵列设施的数据。蛋白质组学试剂合成核心将准备新的荧光试剂，以分析酶活性和翻译后修饰的变化，这是生物刺激所致。 我们将首先支持三位年轻教职员工和一名高级教师的发展来建立关键群众，他们正在研究他的研究新课程。杰出的地方和国家导师以及外部咨询委员会成员将指导这些教师和中心的发展。其次，我们将在建立临界质量的领域聘请四个新教师：化学生物学家/有机合成专家，使用先进的FTMS工具的生物学质谱医师，一种从事代谢建模和代谢组学的系统生物学家，以及使用全球分子工具的细胞生物学家。出色的MSU机构支持（1,400,000美元），蜂窝机制后基因组分析的重要性和及时性以及高级方法中培训的方向将播种新项目，并将带来重大的科学贡献和可持续的中心。