Heart/Cell Function Core

心脏/细胞功能核心

• 批准号: 8299666
• 负责人: Xiongwen Chen
• 金额: $ 22.95万
• 依托单位: TEMPLE UNIV OF THE COMMONWEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8299666
• 关键词: Action Potentials; Adrenergic Agents; Adrenergic Agonists; Aftercare; Animals; Anterior Descending Coronary Artery; Anti-Inflammatory Agents; Anti-inflammatory; Antibodies; Biological Models; Bromodeoxyuridine; Caffeine; Calcium; Cardiac; Cardiac Myocytes; Cell physiology; Cells; Charge; Chronic; Confocal Microscopy; Coronary artery; Coupling; Data; Dependovirus; Development; Diastolic blood pressure; Dobutamine; Echocardiography; Evaluation; Family suidae; Freezing; Head; Health; Heart; Heart failure; Height; Histological Techniques; Infarction; Injury; Instruction; Intraventricular; Ischemia; Isoproterenol; Laboratories; Left; Ligation; Mammals; Measurement; Measures; Membrane; Modality; Modeling; Molecular Analysis; Morphology; Movement; Mus; Muscle Cells; Myocardial Infarction; Myocardium; Operative Surgical Procedures; Pharmaceutical Preparations; Potassium; Program Research Project Grants; Publications; Rattus; Relaxation; Reperfusion Therapy; Research Personnel; Resolution; Rodent; Sampling; Sarcoplasmic Reticulum; Sodium; Stress; Stress Tests; Surface; Techniques; Testing; Tissues; Ventricular; Work; adrenergic; detector; effective therapy; electrical property; gene therapy; heart cell; hemodynamics; implantation; improved; in vivo; indexing; mouse model; osmotic minipump; particle; pressure; programs; therapeutic gene; voltage; voltage clamp

Core C, the Heart/Cell Function Core, is going to serve projects 1-3 in this Program Project Grant to fulfill the following functions: 1. To establish reliable and economical mouse myocardial infarction models by permanently or temporarily ligating the left anterior descending coronary artery. These models will be the flatform for testing the therapeutical treatments in all projects to offer proof-of-concepts for further tests in large mammal (porcine) models; 2. To study in vivo cardiac function changes after Ml injury by echocardiography (weekly) and invasive intraventricular hemodynamic measurements (at the end of each study). These measurements will offer reproducible assessments of the efficacy of all treatments; 3. To isolate and characterize cardiac myocyte function to explore the cellular mechanisms of the potential beneficial effects of the therapeutical modalities as proposed in each project. 4. To centralize tissue and cell samples (mouse and pig) for each project. In this core, we will fix or freeze tissue or isolated cell samples after the treatment is done. These samples will be collected and kept in a centralized and well-maintained facility. The samples will be distributed for each project upon request. 5. To prepare histological samples for all projects. 6. To prepare tissues for molecular analysis and perform these studies when needed. In summary. Core C will provide essential support for all projects in this Project Program Project in an economical while scientifically reliable and efficient way.

核心C，即心脏/细胞功能核心，将服务于本计划项目拨款中的项目1-3，以实现以下功能： 1.通过永久或暂时结扎左冠状动脉前降支的方法建立可靠、经济的小鼠心肌梗死模型。这些模型将成为测试所有项目中治疗方法的平台，为大型哺乳动物（猪）模型的进一步测试提供概念验证； 2.通过超声心动图（每周）和侵入性心室内血流动力学测量（在每个研究结束时）来研究M1损伤后体内心脏功能的变化。这些测量将为所有治疗的功效提供可重复的评估； 3. 分离和表征心肌细胞功能，以探索每个项目中提出的治疗方式的潜在有益效果的细胞机制。 4. 集中每个项目的组织和细胞样本（小鼠和猪）。在此核心中，我们将在治疗完成后固定或冷冻组织或分离的细胞样本。这些样本将被收集并保存在集中且维护良好的设施中。样品将根据要求分发给每个项目。 5. 为所有项目准备组织学样本。 6. 准备用于分子分析的组织并在需要时进行这些研究。 总之。核心C将以经济、科学、可靠和高效的方式为本项目计划项目中的所有项目提供必要的支持。