Microscopy Core

显微镜核心

基本信息

批准号：
8240931
负责人：
Paul S. Cooke
金额：
$ 17.31万
依托单位：
UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-04-01 至 2013-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8240931
关键词：
Achievement Address Autoradiography BMP2 gene CCAAT-Enhancer-Binding Proteins Cells Clinical Core Facility Decidual Cell Reactions Development Diagnostic Disease Embryo Endometrial Endometrium Equipment Faculty Failure Fertility Functional disorder Gel Goals Growth Gynecologic Health Services Accessibility Hormonal Hormones Human Resources Image Immunohistochemistry Implant In Situ Hybridization Individual Infertility Knowledge Laboratories Methodology Methods Microscopic Microscopy Modeling Molecular Nuclear Receptors Paper Pathway interactions Perfusion Procedures Protein Analysis Proteins Regulation Research Research Personnel Role Scientist Screening procedure Services Students Techniques Time Training Vertebral column Woman Work base cost effective endometriosis experience implantation innovation interest light microscopy member multidisciplinary natural Blastocyst Implantation programs sample fixation therapeutic target tool treatment strategy

项目摘要

The overall objective of this U54 Cooperative Specialized Research Center proposal is to characterize, at molecular and cellular levels, the hormonal pathways that regulate embryo implantation and fertility. Failure of the fertilized embryo to implant into the endometrium is a major cause of infertility. The Center will support a multidisciplinary group of scientists with a common interest to understand the mechanisms and cellular pathways that control implantation. The rationale for creating such a Center is to direct our concerted efforts toward the identification of endometrial factors whose aberrant expression or function leads to infertility. The achievement of this goal would require a cohesive, highly effective partnership between the basic and clinical scientists, addressing this question with innovative investigative strategies. This would advance our current understanding of the molecular basis of implantation and help identify factors that underlie infertility in women suffering from endometriosis, a common gynecologic disorder. The knowledge gained from these studies should also aid in developing new molecular diagnostic tools for screening endometrial dysfunction and enable targeted therapeutic strategies for the treatment of infertility. The program is comprised of four complementary, synergistic projects: (1) Role of C/EBP Deta in Uterine Decidualization and Implantation, (2) Nuclear Receptor Coregulators in Implantation and Uterine Function, (3) Regulation of Stromal Differentiation and Implantation by the BMP2 Pathway, and (4) Endometriosis as a Clinical Model of Predecidual Dysfunction. Investigators will be aided by two core facilities: Core A: Administrative Core, and Core B: Microscopy Core.

U54 合作专业研究中心提案的总体目标是描述分子和细胞水平，调节胚胎植入和生育力的激素途径。失败受精胚胎植入子宫内膜是导致不孕的主要原因。该中心将支持由多学科科学家组成的小组，他们对了解机制和细胞有共同的兴趣控制着床的途径。创建这样一个中心的目的是为了指导我们的共同努力旨在识别其异常表达或功能导致不孕的子宫内膜因子。这实现这一目标需要基础和临床之间建立紧密、高效的伙伴关系科学家们通过创新的研究策略来解决这个问题。这将推进我们目前的了解着床的分子基础并帮助识别导致女性不孕的因素患有子宫内膜异位症，这是一种常见的妇科疾病。从这些研究中获得的知识还应有助于开发新的分子诊断工具来筛查子宫内膜功能障碍，并使治疗不孕症的针对性治疗策略。该计划由四个互补、协同的项目组成： (1) C/EBP Deta 在子宫中的作用蜕膜化和着床，(2) 着床和子宫功能中的核受体共调节因子，(3) BMP2 途径对基质分化和植入的调节，以及 (4) 子宫内膜异位症作为一种蜕膜前功能障碍的临床模型。研究人员将得到两个核心设施的帮助：核心 A：管理核心和核心 B：显微镜核心。