Roles and functions of ion channels that mediate mammalian touch transduction.

介导哺乳动物触觉转导的离子通道的作用和功能。

基本信息

批准号：
8296042
负责人：
Diana Michele Bautista
金额：
$ 33.84万
依托单位：
UNIVERSITY OF CALIFORNIA BERKELEY
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-07-01 至 2016-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8296042
关键词：
Acquired Immunodeficiency Syndrome Action Potentials Acute Pain Afferent Neurons Anesthetics Attention Behavior Bladder Capsaicin Carpal Tunnel Syndrome Cells Chronic Development Diabetes Mellitus Diabetic Neuropathies Disease Electrophysiology (science)Environment Esthesia Event Family Glass Goals Heating Human Image In Vitro Individual Inflammation Injury Ion Channel Irritants Lead Libraries Life Link Malignant Neoplasms Mechanical Stimulation Mechanics Mediating Mediator of activation protein Menthol Molecular Molecular Target Mus Mutant Strains Mice Needles Neuroglia Neurons Pain Paresthesia Patients Pharmacology Pharmacotherapy Physiological Physiological Processes Plants Play Radial Reflex action Role Sensory Shapes Signal Pathway Signal Transduction Skin Stimulus Stretching System Tactile Techniques Temperature Testing Texture Tissues Touch sensation Trauma Traumatic Nerve Injury Whole-Cell Recordings base blood pressure regulation cell type cellular imaging cellular targeting chronic pain in vivo insight keratinocyte member mutant nervous system disorder novel pressure receptor research study response sanshool somatosensory tool vibration

项目摘要

DESCRIPTION (provided by applicant): The mammalian somatosensory system detects a wide variety of mechanical stimuli, such as texture, shape, vibration or pressure. This variety of stimuli is matched by a diverse array of mechanosensitive somatosensory neurons. Non-neuronal cells in the skin, such as keratinocytes, may also coordinate with sensory neurons to transduce force. The goal of this proposal is to identify molecular and cellular mechanisms underlying somatosensory mechanotransduction. We hypothesize that both primary afferent somatosensory neurons and keratinocytes mediate mechanosensitive responses in the skin. We will use a variety of techniques including live-cell Ca2+ imaging, electrophysiology and pharmacology to characterize transduction channels in sensory neurons and keratinocytes. We will then probe the role of candidate channels in vivo. The proposed experiments will answer fundamental questions about somatosensory mechanotransduction, including: 1) What are the molecular identities of the ion channels that transduce touch in sensory neurons and keratinocytes? and 2) How does touch-evoked signaling in keratinocytes alter primary afferent neuron function?

描述（由申请人提供）：哺乳动物的体感系统检测到各种机械刺激，例如纹理，形状，振动或压力。这种刺激与各种机械敏感的体感神经元相匹配。皮肤中的非神经元细胞（例如角质形成细胞）也可能与感觉神经元互动以转导力。该提案的目的是确定体感机制转导基础的分子和细胞机制。我们假设一级传入的体感神经元和角质形成细胞都介导皮肤中的机械敏感反应。我们将使用各种技术，包括活细胞Ca2+成像，电生理学和药理学来表征感觉神经元和角质形成细胞中的转导通道。然后，我们将探测候选通道在体内的作用。提出的实验将回答有关体感机械转导的基本问题，包括：1）在感觉神经元和角质形成细胞中传递触摸的离子通道的分子身份是什么？ 2）角质形成细胞中接触的诱发信号如何改变原发性传入神经元功能？