Cell Culture and Molecular Technology Core

细胞培养和分子技术核心

基本信息

批准号：
7665014
负责人：
Pratima Karnik
金额：
$ 10.28万
依托单位：
CASE WESTERN RESERVE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-08-01 至 2011-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7665014
关键词：
Archives Area Arts Basic Science Biology Cell Culture Techniques Cell Line Cells Clinic Collaborations Communities Cost Savings Cultured Cells Dermal Dermatologic Development Disease Doctor of Medicine Doctor of Philosophy Dominant-Negative Mutation Energy Transfer Environment Fibroblasts Fluorescence Foundations Gene Delivery Goals Imaging technology Investigation Maintenance Measures Medical center Methodology Mission Molecular Molecular Biology Patient Care Phosphotransferases Play Proteins Proteomics Quality Control Range Reagent Recording of previous events Recruitment Activity Research Research Activity Research Personnel Resources Role Science Services Signaling Molecule Skin Small Interfering RNA Source Speed Technology Time Training Translations United States Department of Veterans Affairs Universities Vision Work base career cell type cost cost effective epidermis cell experience image visualization improved instrumentation keratinocyte medical schools melanocyte member new technology protein protein interaction response skin disorder vector

项目摘要

The SDRC Cell Culture and Molecular Technology Core (CCMTC) has played an important role in facilitating skin-based research activity at the Case School of Medicine throughout its fifteen year history. During this time, the core has provided basic services and training, and also state-of-the-art services, facilities and expertise. The CCMTC has now developed into a major hub of research activity on campus and has an active role in introducing new investigators to work on dermatological disease. The core is heavily engaged in providing a wide range of cultured skin cells to investigators, and in providing training in cell culture methodology and molecular biology technology. The three major missions of the CCMTC include the following. Specific Aim I A primary goal of the CCMTC is to provide state-of-the-art cell culture and molecular technology services and expertise, and cost savings to the Skin Diseases Research Center (SDRC) membership at the Case Western Reserve University School of Medicine, and to the larger community of researchers affiliated with the Case School of Medicine. The CCMTC has been very successful in supplying investigators with new technology. This remains a core goal of the CCMTC. In response to the demands of a changing scientific environment, the core has historically added new state-ofthe- art technology as it becomes available. In addition to the substantial list of technology that is presently offered, we will now provide access, expertise and training in advanced real-time fluorescent imaging for visualization of intracellular protein-protein interaction (FRET, FRAP, etc.), chip array analysis, and proteomics. Specific Aim 2 A major goal of the CCMTC, in conjunction with the other SDRC cores, is to provide cell and molecular technology, and expertise, that facilitates and encourages the translation of basic science projects to the bedside. A major goal of the SDRC is to serve as a conduit that benefits dermatologic therapy by bringing new diagnoistics and treatments to the bedside with the goal of improving patient care. Specific Aim 3 The third goal of the CCMTC is to promote career development with a goal of retaining talented young Ph.D. and M.D. investigators in the dermatologic sciences. This is achieved by providing training to new investigators and senior investigators in technologies that will facilitate investigation of dermatologic diseases and by providing a forum for discussion of dematologic science.

SDRC 细胞培养和分子技术核心 (CCMTC) 在促进凯斯医学院在其十五年的历史中开展了基于皮肤的研究活动。在此期间当时，核心提供了基本服务和培训，也提供了最先进的服务、设施和专业知识。 CCMTC 现已发展成为校园研究活动的主要中心，并拥有在引入新的研究人员从事皮肤病研究方面发挥积极作用。核心参与度很高为研究人员提供广泛的培养皮肤细胞，并提供细胞培养培训方法论和分子生物学技术。 CCMTC 的三大使命包括：下列的。具体目标 I CCMTC 的主要目标是提供最先进的细胞培养和分子技术服务和专业知识，以及为皮肤病研究中心节省成本 (SDRC) 凯斯西储大学医学院的会员资格，以及更大的会员资格凯斯医学院附属研究人员社区。 CCMTC 非常成功地为调查人员提供了新技术。这仍然是 CCMTC 的核心目标。在为了满足不断变化的科学环境的需求，该核心历来增加了新的最先进的技术艺术技术可用时。除了目前的大量技术之外如果提供的话，我们现在将为以下人员提供高级实时荧光成像的访问、专业知识和培训：细胞内蛋白质-蛋白质相互作用的可视化（FRET、FRAP 等）、芯片阵列分析和蛋白质组学。具体目标 2 CCMTC 与其他 SDRC 核心一起的主要目标是：提供细胞和分子技术以及专业知识，促进和鼓励基础知识的翻译科学项目到床边。 SDRC 的一个主要目标是充当受益者的渠道皮肤科治疗，将新的诊断和治疗方法带到床边，以改善皮肤病病人护理。具体目标 3 CCMTC 的第三个目标是促进职业发展，其目标是留住有才华的年轻博士和皮肤科学领域的医学博士研究人员。这是通过以下方式实现的为新调查员和高级调查员提供促进调查的技术培训皮肤病学并提供讨论皮肤科学的论坛。