Atherosclerosis and Lipoprotein Analysis Core

动脉粥样硬化和脂蛋白分析核心

• 批准号: 8242737
• 负责人: Jonathan D Smith
• 金额: $ 26.11万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8242737
• 关键词: Adventitious Tissue; Aorta; Area; Atherosclerosis; Biological Assay; Blood Vessels; Cells; Collaborations; Core Facility; Data; In Situ; Individual; Inflammatory Response; Laboratories; Learning; Lesion; Lipids; Lipoproteins; Microscope; Mus; Perfusion; Plant Roots; Preparation; Protocols documentation; Saline; Slide; Staining method; Stains; Standardization; Surface; Thoracic aorta; Tissues; Transgenic Mice; abdominal aorta; aortic arch; investigator training; oil red O

leader's approved protocol to: 1) anesthetize the mice, 2) perform saline perfusion, which euthanizes the mouse by exsanguination, 3) dissect the mouse to display the mouse aorta in situ, 4) remove the aorta and trim off adventitial tissue, 5) cut the aorta longitudinally and fix it flattened between two microscope slides, 6) stain the aorta with oil red O, 7) mount the aorta on a microscope slide, and 8) quantify the % surface lesion area in the entire aorta, as well as in the regions of the aortic arch, and thoracic and abdominal aortas. Utilization of the Core Facility

领导批准的方案：1）麻醉小鼠，2） 进行盐水灌注，通过放血使小鼠安乐死，3）解剖小鼠以显示 小鼠主动脉原位，4）取出主动脉并修剪外膜组织，5）纵向切割主动脉并固定 压平在两个显微镜载玻片之间，6）用油红 O 染色主动脉，7）将主动脉安装在显微镜上 幻灯片，8) 量化整个主动脉以及主动脉弓区域的表面病变面积百分比， 以及胸主动脉和腹主动脉。 核心设施的利用