Biomolecule Production

生物分子生产

基本信息

批准号：
8233440
负责人：
ROBIN A ROSS
金额：
$ 70.09万
依托单位：
HARVARD MEDICAL SCHOOL
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-03-01 至 2014-02-28
项目状态：
已结题

项目摘要

As part of the New England Regional Center of Excellence for Biodefense and Emerging Infectious Diseases (NERCE/BEID), the Biomolecule Production Core Laboratory provides services for production of medium to large scale amounts of purified bacterial, mammalian, and viral biomolecules to investigators conducting biodefense research. This core facility is a state-of-the-art laboratory designed to produce both recombinant proteins and native carbohydrate molecules for use in structural analyses and vaccine formulation. This includes, but is not restricted to, investigators in the following categories: those making use of the Proteomics and Small Molecule Screening Core Laboratories who need to produce sufficient quantities of identified proteins to accomplish proposed screens; those developing vaccines containing bacterial carbohydrate components who need large quantities of antigen for analysis in vitro biologic assays and animal studies; and those already expressing target proteins in small scale using recombinant systems who need larger amounts of recombinant protein to expand their research. Investigators provide data demonstrating efficacy of production and purification methods as well as strains or recombinant plasmid preparations where applicable. The laboratory scales-up these protocols for production and purification of 100's of mg of the target biomolecule that is delivered to the investigator with a report outlining the methods used. Since the Biomolecule Production Core opened in 2004, we have collaborated with 19 investigators from 8 research institutions and one company in the production of over 154 different biomolecules. We have conducted 339 fermentation runs for the production of biomolecules, generating 52 Kg of bacterial cells. More than 277 purifications have been completed and we have delivered to investigators 34 Kg of cell paste, 453 mg of purified carbohydrate, and 3.1 g of various purified recombinant protein.

作为新英格兰地区卓越生物陷入困境和新兴传染病的一部分（Nerce/Beid），生物分子生产核心实验室为生产媒介提供服务大量纯化的细菌，哺乳动物和病毒生物分子向进行研究者生物形式研究。该核心设施是一个最先进的实验室，旨在生产重组蛋白质和天然碳水化合物分子用于结构分析和疫苗制剂。这包括但不限于以下类别的调查人员：蛋白质组学和小分子筛选核心实验室，需要产生足够数量的确定蛋白质以完成建议的筛选；那些开发包含细菌的疫苗的人需要大量抗原进行体外生物学测定和动物研究；那些已经使用重组系统以小规模表达靶蛋白的人需要大量的重组蛋白来扩展其研究。调查人员提供数据证明生产和纯化方法以及菌株或重组质粒的功效适用的准备工作。实验室将这些协议扩展为生产和纯化 100毫克的目标生物分子，该分子已递送给研究者，并概述了方法用过的。自2004年生物分子生产核心开业以来，我们与19位调查人员合作来自8家研究机构和一家公司，用于生产154种不同的生物分子。我们有进行了339次发酵，用于生产生物分子，产生52公斤的细菌细胞。已经完成了277次净化，我们已经交付给了34公斤糊的研究人员， 453毫克纯化的碳水化合物和3.1 g的各种纯化的重组蛋白。