A Global Screen for Genes Regulating Cdt1 Turnover In Response To DNA Damage

针对 DNA 损伤调节 Cdt1 更新的基因的全局筛选

• 批准号: 8292212
• 负责人: Malavika Raman
• 金额: $ 5.94万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2013-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8292212
• 关键词: 26S proteasome; ATP phosphohydrolase; Ataxia-Telangiectasia-Mutated protein kinase; Binding; Cancer Biology; Candidate Disease Gene; Cataloging; Catalogs; Cell Cycle; Cell Cycle Proteins; Cell Cycle Regulation; Cells; Checkpoint kinase 1; Chromatin; Chromosome abnormality; Complex; Cullin Proteins; DNA; DNA Binding; DNA Damage; DNA Repair; DNA biosynthesis; DNA lesion; Ensure; Enzymes; Failure; Fire - disasters; G1 Phase; Genes; Genome; Genomic Instability; Genomics; Genotoxic Stress; Health; Homeostasis; In Vitro; Lead; Licensing; Licensing Factor; Ligase; Link; Mammalian Cell; Membrane; Microscopy; Modeling; Molecular; Mutagens; Mutation; Organism; Pathway interactions; Phase; Phosphotransferases; Play; Proteins; Proteolysis; Reaction; Recruitment Activity; Regulation; Replication Initiation; Replication Licensing; Replication Origin; Role; S Phase; Signal Pathway; Signal Transduction; Small Interfering RNA; System; Techniques; Testing; Transducers; Ubiquitin; Ubiquitination; Work; Xenopus; base; chromatin protein; cofactor; egg; genetic regulatory protein; genome wide association study; genome-wide; insight; loss of function; multicatalytic endopeptidase complex; mutant; novel; prevent; protein complex; protein degradation; receptor; reconstitution; response; sensor; tumorigenesis; ubiquitin ligase; ubiquitin-protein ligase

DESCRIPTION (provided by applicant): DNA replication is a crucial step in the cell cycle where cellular DNA must be faithfully copied only once. Failure to do so will result in either incomplete replication or multiple rounds of replication, both of which result in aberrant chromosome numbers. This proposal aims to understand how a protein known as Cdt1, which has been shown in all organisms to be the key player in controlling DNA replication, is regulated. Mis-regulation of Cdt1 levels plays a key role in tumorigenesis, underscoring the importance of this pathway in cancer biology.

描述（由申请人提供）：DNA 复制是细胞周期中的关键步骤，其中细胞 DNA 必须忠实地复制一次。如果不这样做，将导致复制不完整或多轮复制，这两种情况都会导致染色体数量异常。该提案旨在了解一种名为 Cdt1 的蛋白质是如何受到调控的，该蛋白质已在所有生物体中被证明是控制 DNA 复制的关键参与者。 Cdt1 水平的错误调节在肿瘤发生中起着关键作用，强调了该通路在癌症生物学中的重要性。