OTHER FUNCTIONS: TOPIC 287, PHASE II "NANOTECHNOLOGY SENSING PLATFORMS"

其他功能：主题 287，第二阶段“纳米技术传感平台”

• 批准号: 8557600
• 负责人: SWAPNAJIT CHAKRAVARTY
• 金额: $ 100万
• 依托单位: OMEGA OPTICS, INC.
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-28 至 2014-09-27
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8557600
• 关键词: Antibodies; Area; Automation; Binding; Biological Assay; Breast Cancer Cell; Breast Cancer Detection; Cancer Center; Cancer Detection; Cells; Collaborations; Commit; Correlation Studies; Coupled; Coupling; Data; Data Analyses; Devices; Ensure; Food; Ink; Label; Malignant neoplasm of lung; Measurement; Medical; Methods; Modeling; Nanotechnology; Optics; Ownership; Phase; Printing; Protein Array; Reaction; Reporting; Sampling; Screening for cancer; Sensitivity and Specificity; Silicon; South Carolina; Specificity; Surface; Testing; Time; Universities; Validation; Western Blotting; blind; cost; drug discovery; optical fiber; photonics; sensor

Label-free, highly sensitive early cancer detection sensors with required specificity are in great demand. In Phase 1, we experimentally confirmed feasibility of our proposed photonic crystal (PC) microarray with real data better than all reported sensing methods. These include absolute biomolecule mass sensitivity 8.8attograms (10A-18grams), surface mass sensitivity 0.8picogram (10A-12grams)/mmA2 in a sensing area 5.7micronA2. We demonstrated sensing capability of 72 binding reactions between a target and its conjugate probe biomolecule simultaneously, at the same time with 60 microliters total probe sample volume. We detected lung cancer ceillysates with specificity by sandwich assay. In Phase 2, all demonstrated components for chip integrated microarray will be integrated with antibodies detecting lung and breast cancer cell Iysates with high sensitivity, specificity and throughput via committed collaborations with Medical University of South Carolina and MD Anderson Cancer Center. The device has biomolecule functionalized PC microcavity resonator arrays coupled to a PC waveguide, further arrayed using multimode interference splitters, which transduce specific binding between probe and target biomolecules. Our multi-analyte sensing has applications in drug discovery, food feedstock assays and biD-warfare defense. Silicon CMOS compatibility ensures mass manufacturability with at least one order of magnitude better cost of ownership than any existing approach

具有所需特异性的无标记、高灵敏度早期癌症检测传感器的需求量很大。在第一阶段，我们 实验证实了我们提出的光子晶体（PC）微阵列的可行性，真实数据优于所有微阵列 报道了传感方法。其中包括绝对生物分子质量灵敏度 8.8 阿克（10A-18 克）、表面 质量灵敏度为 0.8 皮克（10A-12 克）/mmA2，传感区域为 5.7 微米 A2。我们展示了传感能力 目标与其共轭探针生物分子之间同时进行 72 个结合反应，同时进行 60 个结合反应 微升探针样品总体积。我们通过夹心法特异性检测肺癌细胞裂解液。 在第二阶段，芯片集成微阵列的所有演示组件将与检测肺部的抗体集成 通过与 Medical 的坚定合作，获得具有高灵敏度、特异性和通量的乳腺癌细胞裂解物 南卡罗来纳大学和 MD 安德森癌症中心。该装置具有生物分子功能化PC 耦合到 PC 波导的微腔谐振器阵列，使用多模干涉分离器进一步排列， 转导探针和目标生物分子之间的特异性结合。我们的多分析物传感在药物领域有应用 发现、食品原料分析和双向战防御。硅 CMOS 兼容性确保了大规模可制造性 拥有成本比任何现有方法至少低一个数量级