Pathology Core for NF1 Research

NF1 研究的病理学核心

基本信息

批准号：
8382458
负责人：
Tilat Rizvi
金额：
$ 10.66万
依托单位：
CINCINNATI CHILDRENS HOSP MED CTR
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至 2014-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8382458
关键词：
Aftercare Anatomy Animal Organ Animals Antibodies Antigens Apoptosis Arts Autopsy Bar Codes Biological Brain Neoplasms Cell Count Charge Childhood Consult Core Facility Data Databases Diagnosis Dissection Educational process of instructing Electron Microscope Electron Microscopy Electrons Epidermal Growth Factor Receptor Epitopes Failure Genomics Goals Growth Histology Human Human Resources Immunohistochemistry Individual Informatics Joint Ventures Laboratories Letters Life Link Mails Methods Microscopic Minnesota Mus Nerve Nervous System Neoplasms Neuroanatomy Neurofibromatoses Online Systems Paraffin Embedding Pathologic Pathologist Pathology Pediatric Hospitals Perfusion Peripheral Nerves Plastic Embedding Postdoctoral Fellow Process Protocols documentation Reagent Research Research Activity Research Institute Research Personnel Retrieval Services Site Slide Specimen Staining method Stains Support System Synaptophysin System Text Therapeutic Agents Time Tissue Sample Tissue Stains Tissues Toxic effect Training Universities Work Xenograft procedure base cost data sharing digital imaging experience mast cell member paraform sample fixation success tissue fixing tumor tumor xenograft

项目摘要

We propose to facilitate analysis of nervous system tumors in this group effort by providing centralized mouse pathology services, taking advantage of local expertise in mouse perfusion and analysis of mouse nerve and brain tumors. This Core facility will use components of a highly successful core laboratory located within the Department of Pathology at Cincinnati Children's Hospital for paraffin embedding, plastic embedding for EM and electron microscope beam time. The pathology core will serve as an essential component for the research activities at Cincinnati Children's Hospital and will provide services to 3 investigators from Cincinnati Children's Hospital, University of Cincinnati Genomic research Institute (GRI),and University of Minnesota. The specific aims of this core are to 1) provide investigators assistance in tissue based approaches for nerve histology, immunohistochemistry, and electron microscopy; 2) provide pathologic support for non-perfused tissue such as tumors from xenografts, with support from human pathologists.

我们建议通过提供小鼠灌注和小鼠神经和脑肿瘤分析的局部专业知识，通过提供集中的小鼠病理学服务来促进该组努力中神经系统肿瘤的分析。该核心设施将使用位于辛辛那提儿童医院病理学系内的非常成功的核心实验室的组件，用于塑料嵌入EM和电子显微镜光束时间。病理核心将成为辛辛那提儿童医院研究活动的重要组成部分，并将为辛辛那提儿童医院，辛辛那提大学基因组研究所（GRI）和明尼苏达大学的3名调查人员提供服务。该核心的具体目的是1）在基于组织的组织学，免疫组织化学和电子显微镜的基于组织的方法方面提供帮助； 2）提供在人类病理学家的支持下，对非精液组织（例如异种移植物的肿瘤）的病理支持。