BIOLOGICAL TESTING CORE

生物检测核心

基本信息

批准号：
7384101
负责人：
ALEX S. EVERS
金额：
$ 12.37万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-08-01 至 2012-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7384101
关键词：
Allopregnanolone Alphaxolone Anesthesia procedures Anesthesiology Anesthetics Animals Barbiturates Behavioral Assay Binding Biological Assay Biological Testing British Cells Classification Complex Core Facility Data Databases Dependence Drug effect disorder Equipment Evaluation Filtration GABA Receptor GABA-A Receptor General Anesthesia General anesthetic drugs Hydrostatic Pressure Individual Ion Channel Journals Literature Membrane Methohexital Molecular Molecular Target Mus Neurons Numbers Pharmacology Physiological Pregnanolone Preparation Production Progress Reports Propofol Publications Rattus Reflex action Relative (related person)Research Research Activity Role Science Scintillation Counter Site Speed Spinal Cord Steroids Structure-Activity Relationship Tadpoles Testing Therapeutic Wood material Yang alphadolone analog barbituric acid salt drug structure frontal lobe gamma-Aminobutyric Acid molecular site neurochemistry pressure programs receptor receptor binding response steroid hormone tert-butylbicyclophosphorothionate

项目摘要

for use. The Biological Testing Core Facility is fully operational at present. We have acquired the necessary filtration apparatus, testing equipment for tadpoles and equipment for membrane preparations. The Research Unit shared facilities will be freely available for use, including centrifuges (low-, intermediate- and high-speed), cold room, gamma and scintillation counters and freezers. 2.F. LITERATURE CITED FOR THE BIOLOGICAL TESTING CORE FACILITY. [1] Covey, DF, Nathan, D, Kalkbrenner, M, Nilsson, KR, Hu, Y, Zorumski, CF and Evers, AS, Enantioselectivity of pregnanolone-induced gamma-aminobutyric acid(A) receptor modulation and anesthesia. Journal of Pharmacology and Experimental Therapeutics. 293:1009-1016, 2000. [2] Franks, NP and Lieb, WR, Selective actions of volatile general anaesthetics at molecular andcellular levels. British Journal of Anaesthesia. 71:65-76, 1993. [3] Gee, KW and Lan, NC, Gamma-aminobutyric acidA receptor complexes in rat frontal cortex and spinal cord show differential responses to steroid modulation. Molecular Pharmacology. 40:995- 999, 1991. [4] Halsey, MJ, Wardley-Smith, B and Wood, S, Pressure reversal of alphaxalone/alphadolone and methohexitone in tadpoles: evidence for different molecular sites for general anaesthesia. British Journal of Pharmacology. 89:299-305, 1986. [5] Majewska, MD, Harrison, NL, Schwartz, RD, Barker, JL and Paul, SM, Steroid hormone metabolites are barbiturate-like modulators of the GABA receptor. Science. 232:1004-1007, 1986. [6] Paradiso, K, Sabey, K, Evers, AS, Zorumski, CF, Covey, DF and Steinbach, JH, Steroid inhibition of rat neuronal nicotinic a4 B2 receptors expressed in HEK 293 cells. Molecular Pharmacology. 58:341-351,2000. [7] Srinivasan, S, Sapp, DW, Tobin, AJ and Olsen, RW, Biphasic modulation of GABA receptor binding by steroids suggests functional correlates. Neurochemical Research. 24:1363-1372, 1999. [8] Tonner, PH, Poppers, DM and Miller, KW, The general anesthetic potency of propofol and its dependence on hydrostatic pressure. Anesthesiology. 77:926-931, 1992. [9] Turner, DM, Ransom, RW, Yang, JS and Olsen, RW, Steroid anesthetics and naturally occurring analogs modulate the gamma-aminobutyric acid receptor complex at a

以供使用。生物检测核心设施全面投入运营现在。我们拥有必要的蝌蚪过滤设备、检测设备和膜制备设备。研究单位共享设施将免费使用，包括离心机（低速、中速和高速）、冷藏室、伽马计数器和闪烁计数器和冰柜。 2.F.生物测试核心设施引用的文献。 [1] Covey, DF、Nathan, D、Kalkbrenner, M、Nilsson, KR、Hu, Y、Zorumski, CF 和 Evers, AS，孕烯醇酮诱导的γ-氨基丁酸(A)受体调节的对映选择性麻醉。药理学和实验治疗学杂志。 293：1009-1016，2000。 [2] Franks, NP 和 Lieb, WR，挥发性全身麻醉剂对分子和细胞的选择性作用水平。英国麻醉杂志。 71:65-76, 1993。 [3] Gee, KW 和 Lan, NC，大鼠额叶皮质中的 γ-氨基丁酸 A 受体复合物和脊髓对类固醇调节表现出不同的反应。分子药理学。 40:995- 999、1991。 [4] Halsey, MJ、Wardley-Smith, B 和 Wood, S，alphaxalone/alphadolone 的压力逆转和蝌蚪中的甲己西酮：全身麻醉不同分子位点的证据。英国人药理学杂志。 89：299-305，1986 年。 [5] Majewska，MD，Harrison，NL，Schwartz，RD，Barker，JL 和 Paul，SM，类固醇激素代谢物是 GABA 受体的巴比妥类调节剂。科学。 232：1004-1007，1986 年。 [6] Paradiso, K, Sabey, K, Evers, AS, Zorumski, CF, Covey, DF 和 Steinbach, JH，类固醇抑制 HEK 293 细胞中表达的大鼠神经元烟碱 a4 B2 受体。分子药理学。 58：341-351，2000。 [7] Srinivasan, S, Sapp, DW, Tobin, AJ 和 Olsen, RW，GABA 受体结合的双相调节类固醇表明功能相关。神经化学研究。 24：1363-1372，1999。 [8] Tonner, PH、Poppers, DM 和 Miller, KW，异丙酚的全身麻醉效力及其依赖于静水压力。麻醉学。 77：926-931，1992 年。 [9] Turner, DM, Ransom, RW, Yang, JS 和 Olsen, RW，类固醇麻醉剂和天然存在的类似物调节 γ-氨基丁酸受体复合物