Proteomics and Genomics Core

蛋白质组学和基因组学核心

• 批准号: 8324850
• 负责人: Daniel R. Salomon
• 金额: $ 34.04万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-15 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8324850
• 关键词: Allografting; Bioinformatics; Biological; Biological Markers; Biotechnology; Blood Cells; California; Cell Separation; Cells; Clinical Trials; Collaborations; Complex; Core Facility; DNA; DNA Microarray Chip; DNA Sequencing Facility; Doctor of Philosophy; Event; Exons; Faculty; Failure; Funding; Gene Expression; Genes; Genome; Genomics; Grant; Head; Human; Immune response; Informatics; Infusion procedures; Institutes; Instruction; Islets of Langerhans Transplantation; Kidney Transplantation; Laboratories; Leadership; Letters; Mass Spectrum Analysis; Medicine; Mesenchymal Stem Cell Transplantation; Mesenchymal Stem Cells; Messenger RNA; Metric; Modeling; Molecular; Nucleic Acids; Outcome; Principal Investigator; Proteins; Proteomics; Protocols documentation; RNA; RNA Sequences; Recording of previous events; Regulation; Reperfusion Injury; Research; Research Institute; Research Personnel; Resources; Sampling; Services; Specialist; Staging; Structure; Supervision; Technology; Time; Tissues; Translational Research; Translations; Transplantation; United States National Institutes of Health; Universities; Work; base; design; experience; functional genomics; mass spectrometer; next generation; nonhuman primate; programs; regenerative; response; square foot; success; tandem mass spectrometry

PROJECT SUMMARY (See instructions): The Genomics and Proteomics Core will be composed of six functional sub-cores located within The Scripps Research Institute. Each sub-core is organized with a clear leadership structure and they are designed to be functionally complementary to the Program's objectives: Core 1 - Sample Receiving Center Core 2 - Gene Expression Microarrays Core 3 - Next Generation Sequencing Core 4 - Quantitative PCR Core 5 - Mass Spectrometry Proteomics Core 6 - Genomic Informatics The primary objectives of the Genomics and Protemics Core will be to provide the investigators in Projects 1 and 2 with cutting edge technologies and supportive expertise for functional genomics including DNA microarrays, tandem mass spectrometry proteomics and next generation deep RNA sequencing. These technologies will be applied to parallel studies of carefully designed nonhuman primate models of islet and kidney transplantation. Experimentally-defined transplant outcomes will be the focus including the activation of mesenchymal stem cells (MSC), graft infiltrating cells during different stages of rejection or successful tolerance, and peripheral blood cells and constituent subsets of biological significance such as Tregs as a function of time after transplantation and MSC infusions and with success or failure. Finally, the Core will develop a new nonhuman primate DNA microarray with Affymetrix based on the latest exon probe technology that will advance the present work and provide the entire field with a new resource. The ultimate objective is to support the translation of a successful and optimized protocol for MSC-based transplantation therapy into a human clinical trial, creating genomic metrics for high quality MSC isolations and immunological biomarkers for the impact of MSC on the transplant immune response

项目摘要（请参阅说明）： 基因组学和蛋白质组学核心将由位于Scripps研究所内的六个功能子核组成。每个子核心都有明确的领导结构组织，它们旨在与该计划的目标互补： 核心1-样品接收中心 核心2-基因表达微阵列 核心3-下一代测序 核心4-定量PCR 核心5-质谱蛋白质组学 核心6-基因组信息学 基因组学和原始物质核心的主要目标是为项目1和2的研究人员提供尖端技术和功能基因组学的支持性专业知识，包括DNA微阵列，串联质谱蛋白质组学和下一代深层RNA测序。这些技术将应用于智能设计的胰岛和肾脏移植的非人类灵长类动物模型的平行研究。实验定义的移植结局将是重点，包括间充质干细胞（MSC）的激活，在不同的排斥或成功耐受阶段，移植物浸润细胞以及外周血细胞和生物学意义的成分子集，例如TREG作为TREG作为时间功能的生物学意义移植和MSC输注后并取得成功或失败。最后，核心将基于最新的外显子探测技术开发一种新的非人类灵长类动物DNA微阵列，并使用Affymetrix开发出来，该技术将推进目前的工作，并为整个领域提供新的资源。最终目标是支持将基于MSC的移植疗法成功，优化的方案转换为人类临床试验，从而为高质量MSC隔离和免疫生物标志物创建基因组指标，以影响MSC对移植免疫反应的影响