SEMEN COLLECTION FROM MALE BABOONS

雄性狒狒的精液采集

• 批准号: 8172723
• 负责人: GERALD SCHATTEN
• 金额: $ 29.1万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172723
• 关键词: Antibodies; Area; Assisted Reproductive Technology; Autopsy; Biochemistry; Cell Line; Coagulation Process; Collection; Computer Retrieval of Information on Scientific Projects Database; Cryopreservation; Development; Edetic Acid; Ejaculation; Female; Fertilization; Fertilization in Vitro; Funding; Genotype; Grant; Institution; Investigation; Liquid substance; Macaca; Macaca mulatta; Monitor; Nitrogen; Oocytes; Other Genetics; Ovary; Pan Genus; Papio; Procedures; Punch Biopsy; Research; Research Personnel; Resources; Rest; Retrieval; Sampling; Seminal fluid; Skin; Source; Sperm Banks; Stem Cell Research; Stem cells; Testing; Tube; United States National Institutes of Health; Veterinarians; Whole Blood; established cell line; immunocytochemistry; male; rectal; reproductive; sperm cell; sperm quality

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Rectal probe electro-ejaculation of male baboons has proven reliable for producing fresh, high quality sperm samples for investigative use in assisted reproductive technologies (ART) studies. In order to obtain baboon sperm samples for developmental reproductive studies and stem cell research, assigned male baboons provided semen samples via rectal probe electro-ejaculation for sperm isolation. Five identified areas for the use of baboon sperm include: 1. In vitro fertilizations of oocytes collected via follicular aspirations from hormonally stimulated female baboons. (Sperm required day of aspiration procedures; intra-species fertilizations) 2. In vitro fertilizations of oocytes from unplanned necropsy ovaries. (Sperm required 48hr from oocyte isolations on necropsy date; intra & inter-species fertilizations) 3. Alternative sperm source for fertilization studies in either macaques (rhesus; cynomolgus) or chimpanzee oocytes when neither macaque nor chimpanzee sperm is unattainable on day of oocyte retrieval. (Sperm required day of oocyte collections; inter-species fertilizations) 4. Cryopreservation and banking of sperm in liquid nitrogen. 5. Fresh sperm collections for research investigations (i.e. testing new antibodies [immunocytochemistry]; biochemistry studies; etc). The requested baboons will be allowed rest periods between ejaculation procedures as mandated by the current facility standard operating procedure and monitored by a veterinarian. For males that successfully produce stem cell lines, we may request 2-3 skin samples (~5mm biopsy punch), 3-5mls of whole blood in clot tube and/or 3-5mls of whole blood in EDTA tube for genotyping or other genetic comparisons between the male and the established cell lines.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 事实证明，雄性狒狒的直肠探针电射精可以可靠地产生新鲜、高质量的精子样本，用于辅助生殖技术 (ART) 研究。为了获得狒狒精子样本用于发育生殖研究和干细胞研究，指定的雄性狒狒通过直肠探针电射精提供精液样本以进行精子分离。 狒狒精子的五个确定的使用领域包括： 1.通过从受激素刺激的雌性狒狒的卵泡抽吸收集的卵母细胞进行体外受精。 （精子所需的抽吸程序日；物种内受精） 2.来自非计划尸检卵巢的卵母细胞的体外受精。 （在尸检日期，精子需要 48 小时从卵母细胞分离；种内和种间受精） 3. 当在卵母细胞取出当天无法获得猕猴或黑猩猩精子时，可在猕猴（恒河猴；食蟹猴）或黑猩猩卵母细胞中进行受精研究的替代精子来源。 （精子采集卵母细胞所需的天数；种间受精） 4. 液氮中精子的冷冻保存和储存。 5. 用于研究调查的新鲜精子采集（即测试新抗体[免疫细胞化学]；生物化学研究等）。 根据当前设施标准操作程序的规定，将允许所请求的狒狒在射精程序之间休息一段时间，并由兽医进行监控。对于成功产生干细胞系的男性，我们可能会要求 2-3 个皮肤样本（~5mm 活检穿孔）、凝块管中的 3-5 毫升全血和/或 EDTA 管中的 3-5 毫升全血用于基因分型或其他遗传分析雄性细胞系和已建立的细胞系之间的比较。